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Infection and Immunity, May 2001, p. 2928-2934, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2928-2934.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Oral Immunization with Recombinant Streptococcus gordonii Expressing Porphyromonas gingivalis FimA Domains

Ashu Sharma,1,* Kiyonobu Honma,1 Richard T. Evans,1,2 Dennis E. Hruby,3 and Robert J. Genco1,2

Department of Oral Biology, School of Dental Medicine,1 and Department of Microbiology, School of Medicine and Biomedical Sciences,2 State University of New York at Buffalo, Buffalo, New York 14214, and Department of Microbiology, Oregon State University, Corvallis, Oregon 973313

Received 31 August 2000/Returned for modification 30 October 2000/Accepted 21 February 2001

Porphyromonas gingivalis, a gram-negative anaerobe, is implicated in the etiology of adult periodontitis. P. gingivalis fimbriae are one of several critical surface virulence factors involved in both bacterial adherence and inflammation. P. gingivalis fimbrillin (FimA), the major subunit protein of fimbriae, is considered an important antigen for vaccine development against P. gingivalis-associated periodontitis. We have previously shown that biologically active domains of P. gingivalis fimbrillin can be expressed on the surface of the human commensal bacterium Streptococcus gordonii. In this study, we examined the effects of oral coimmunization of germfree rats with two S. gordonii recombinants expressing N (residues 55 to 145)- and C (residues 226 to 337)-terminal epitopes of P. gingivalis FimA to elicit FimA-specific immune responses. The effectiveness of immunization in protecting against alveolar bone loss following P. gingivalis infection was also evaluated. The results of this study show that the oral delivery of P. gingivalis FimA epitopes via S. gordonii vectors resulted in the induction of FimA-specific serum (immunoglobulin G [IgG] and IgA) and salivary (IgA) antibody responses and that the immune responses were protective against subsequent P. gingivalis-induced alveolar bone loss. These results support the potential usefulness of the S. gordonii vectors expressing P. gingivalis fimbrillin as a mucosal vaccine against adult periodontitis.

* Corresponding author. Mailing address: Department of Oral Biology, 211 Foster Hall, 3435 Main St., State University of New York at Buffalo, Buffalo, NY 14214. Phone: (716) 829-2758. Fax: (716) 829-3942. E-mail: sharmaa{at}buffalo.edu.

Infection and Immunity, May 2001, p. 2928-2934, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2928-2934.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.