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Infection and Immunity, May 2001, p. 2935-2942, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2935-2942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effective In Vitro Clearance of Porphyromonas gingivalis by Fcalpha Receptor I (CD89) on Gingival Crevicular Neutrophils

Tetsuo Kobayashi,1,* Kouji Yamamoto,1 Noriko Sugita,1 Annemiek B. van Spriel,2 Susumu Kaneko,1 Jan G. J. van de Winkel,2,3 and Hiromasa Yoshie1

Department of Periodontology, Faculty of Dentistry, Niigata University, Niigata, Japan,1 and Department of Immunology2 and Genmab,3 University Medical Center Utrecht, Utrecht, The Netherlands

Received 7 September 2000/Returned for modification 1 December 2000/Accepted 5 February 2001

Porphyromonas gingivalis has been implicated as a causative pathogen in periodontitis. Immunotherapeutic approaches have recently been suggested to aid in the clearance of P. gingivalis from disease sites. Because antibody-Fc receptor (FcR) interactions play a role in the effector functions of polymorphonuclear neutrophils (PMN), we evaluated which FcR on PMN from gingival crevicular fluid (GCF) serves as an optimal target molecule for FcR-directed immunotherapy. GCF PMN and peripheral blood (PB) PMN from adult periodontitis patients were analyzed for their immunoglobulin G (IgG) and IgA FcR (Fcgamma R and Fcalpha R, respectively) expression and function by studying IgG- and IgA-mediated elimination of P. gingivalis. GCF PMN exhibited higher Fcalpha RI and Fcgamma RI levels and lower Fcgamma RIIa and Fcgamma RIIIb levels than PB PMN. Functional studies revealed that GCF PMN exhibited less of a capacity to phagocytose and kill IgG1-opsonized P. gingivalis than PB PMN. IgA1-mediated phagocytosis and killing capacity was, however, comparable between GCF PMN and PB PMN. In summary, these in vitro results document that Fcalpha RI represents a candidate target for FcR-directed immunotherapy for the clearance of P. gingivalis.

* Corresponding author. Mailing address: Department of Periodontology, Faculty of Dentistry, Niigata University, Gakko-cho 2-5274, 951-8514 Niigata, Japan. Phone: (81) 25-227-2871. Fax: (81) 25-227-0808. E-mail: kotetsuo{at}dent.niigata-u.ac.jp.

Infection and Immunity, May 2001, p. 2935-2942, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2935-2942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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