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Infection and Immunity, May 2001, p. 3128-3134, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3128-3134.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Protective Efficacy of H Antigen from Histoplasma capsulatum in a Murine Model of Pulmonary Histoplasmosis

George S. Deepe Jr.* and Reta Gibbons

Division of Infectious Diseases, University of Cincinnati College of Medicine, and Cincinnati Veterans Affairs Hospital, Cincinnati, Ohio

Received 8 December 2000/Returned for modification 24 January 2001/Accepted 31 January 2001

We previously reported that immunization with H antigen from Histoplasma capsulatum did not protect mice against an intravenous challenge with yeasts. Here, we investigated the utility of H antigen to protect mice in a model of pulmonary histoplasmosis. Mice immunized with H antigen and challenged intranasally 4 weeks postvaccination were protected against sublethal and lethal challenges with H. capsulatum yeasts. If the challenge was performed 3 months after vaccination, there was a reduction in fungal burden following sublethal challenge and a modest delay in mortality in mice given a lethal inoculum. Vaccination was associated with production of gamma interferon, granulocyte-macrophage colony-stimulating factor, interleukin-4, and interleukin-10 by splenocytes. Vaccination with H antigen was not accompanied by a major expansion of CD4+ or CD8+ cells in spleens of mice. These results demonstrate that H antigen may be useful as a protective immunogen against pulmonary exposure to H. capsulatum.

* Corresponding author. Mailing address: Division of Infectious Diseases, Dept. of Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0560. Phone: (513) 558-4704. Fax: (513) 558-2089. E-mail: george.deepe{at}uc.edu.

Infection and Immunity, May 2001, p. 3128-3134, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3128-3134.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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