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Infection and Immunity, May 2001, p. 3435-3437, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3435-3437.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

PspC, a Pneumococcal Surface Protein, Binds Human Factor H

Sandhya Dave,1 Alexis Brooks-Walter,2,dagger Michael K. Pangburn,3 and Larry S. McDaniel1,4,5,*

Departments of Microbiology,1 Surgery,4 and Medicine,5 The University of Mississippi Medical Center, Jackson, Mississippi 39216; Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 352942; and Department of Biochemistry, The University of Texas Health Science Center, Tyler, Texas 757083

Received 27 September 2000/Returned for modification 15 November 2000/Accepted 17 January 2001

PspC was found to bind human complement factor H (FH) by Western blot analysis of D39 (pspC+) and an isogenic mutant TRE108 (pspC). We confirmed that PspA does not bind FH, while purified PspC binds FH very strongly. The binding of FH to exponentially growing pneumococci varied among different isolates when analyzed by fluorescence activated cell sorting analysis.

* Corresponding author. Mailing address: The University of Mississippi Medical Center, 2500 North State St., Jackson, MS 39216. Phone: (601) 984-6880. Fax: (601) 984-1708. E-mail: LMcDaniel{at}microbio.umsmed.edu.

dagger Present address: Department of Biology, Florida A&M University, Tallahassee, FL 32307.

Infection and Immunity, May 2001, p. 3435-3437, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3435-3437.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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