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Infection and Immunity, May 2001, p. 3442-3446, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3442-3446.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular and Genomic Analysis of Genes Encoding Surface-Anchored Proteins from Clostridium difficile

Tuomo Karjalainen,1 Anne-Judith Waligora-Dupriet,1 Marina Cerquetti,2 Patrizia Spigaglia,2 Andrea Maggioni,3 Pierluigi Mauri,3 and Paola Mastrantonio2,*

Département de Microbiologie, Faculté de Pharmacie, Université de Paris-Sud, 92296 Châtenay-Malabry Cedex, France,1 and Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, Rome 00161,2 and Istituto Tecnologie Biomediche Avanzate/CNR, 20090 Segrate-Milan,3 Italy

Received 27 November 2000/Returned for modification 30 January 2001/Accepted 8 February 2001

The gene slpA, encoding the S-layer precursor protein in the virulent Clostridium difficile strains C253 and 79-685, was identified. The precursor protein carries a C-terminal highly conserved anchoring domain, similar to the one found in the Cwp66 adhesin (previously characterized in strain 79-685), an SLH domain, and a variable N-terminal domain mediating cell adherence. The genes encoding the S-layer precursor proteins and the Cwp66 adhesin are present in a genetic locus carrying 17 open reading frames, 11 of which encode a similar two-domain architecture, likely to include surface-anchored proteins.

* Corresponding author. Mailing address: Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, Viale Regina Elena 299, Rome 00161, Italy. Phone: 39-06 49902335. Fax: 39-06 49387112. E-mail: pmastran{at}iss.it.

Infection and Immunity, May 2001, p. 3442-3446, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3442-3446.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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