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Infection and Immunity, May 2001, p. 3460-3465, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3460-3465.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Assessing Vascular Permeability during Experimental Cerebral Malaria by a Radiolabeled Monoclonal Antibody Technique

Henri C. van der Heyde,1,2,* Philippe Bauer,3 Guang Sun,2 Wun-Ling Chang,4 Lijia Yin,4 John Fuseler,4,5 and D. Neil Granger3

Departments of Microbiology and Immunology,1 Cellular and Molecular Physiology,3 and Medicine,4 Inflammation and Immunology Research Group,2 and Center for Excellence in Arthritis and Rheumatism,5 Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130

Received 3 August 2000/Returned for modification 27 September 2000/Accepted 29 January 2001

Vascular endothelial integrity, assessed by Evans blue dye extrusion and radiolabeled monoclonal antibody leakage, was markedly compromised in the brain, lung, kidney, and heart during Plasmodium berghei infection, a well-recognized model for human cerebral malaria. The results for vascular permeability from both methods were significantly (P < 0.001) related.

* Corresponding author. Mailing address: LSU Health Sciences Center---Shreveport, Department of Microbiology and Immunology, P.O. Box 33932, Shreveport, LA 71130. Phone: (318) 675-4457. Fax: (318) 675-5764. E-mail: hvande{at}lsuhsc.edu.

Infection and Immunity, May 2001, p. 3460-3465, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3460-3465.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.