This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reddy, K.
Right arrow Articles by Ross, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reddy, K.
Right arrow Articles by Ross, J. M.

 Previous Article  |  Next Article 

Infection and Immunity, May 2001, p. 3472-3475, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3472-3475.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Shear Stress Prevents Fibronectin Binding Protein-Mediated Staphylococcus aureus Adhesion to Resting Endothelial Cells

Kesav Reddy and Julia M. Ross*

Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, Maryland 21250

Received 5 October 2000/Returned for modification 22 November 2000/Accepted 7 February 2001

Fibronectin binding proteins (FnBP) on the surface of Staphylococcus aureus have previously been shown to mediate adherence of the organism to resting endothelial cells in static adhesion assays. However, in this study using well-defined flow assays, we demonstrate that physiologic levels of shear stress prevent FnBP-mediated adhesion of S. aureus 8325-4 to resting endothelial cells. This result suggests that mechanical forces present in vivo may influence the ability of staphylococci to bind endothelial cell surfaces.

* Corresponding author. Mailing address: Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250. Phone: (410) 455-3414. Fax: (410) 455-1049. E-mail: jross{at}umbc.edu.

Infection and Immunity, May 2001, p. 3472-3475, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3472-3475.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kerrigan, S. W., Clarke, N., Loughman, A., Meade, G., Foster, T. J., Cox, D. (2008). Molecular Basis for Staphylococcus aureus-Mediated Platelet Aggregate Formation Under Arterial Shear In Vitro. Arterioscler. Thromb. Vasc. Bio. 28: 335-340 [Abstract] [Full Text]  
  • Asai, T., Lee, M.-H., Arrecubieta, C., von Bayern, M. P., Cespedes, C. A., Baron, H. M., Cadeiras, M., Sakaguchi, T., Marboe, C. C., Naka, Y., Deng, M. C., Lowy, F. D. (2007). Cellular coating of the left ventricular assist device textured polyurethane membrane reduces adhesion of Staphylococcus aureus. J. Thorac. Cardiovasc. Surg. 133: 1147-1153 [Abstract] [Full Text]  
  • Hall-Stoodley, L., Watts, G., Crowther, J. E., Balagopal, A., Torrelles, J. B., Robison-Cox, J., Bargatze, R. F., Harmsen, A. G., Crouch, E. C., Schlesinger, L. S. (2006). Mycobacterium tuberculosis Binding to Human Surfactant Proteins A and D, Fibronectin, and Small Airway Epithelial Cells under Shear Conditions.. Infect. Immun. 74: 3587-3596 [Abstract] [Full Text]  
  • Pawar, P., Shin, P. K., Mousa, S. A., Ross, J. M., Konstantopoulos, K. (2004). Fluid Shear Regulates the Kinetics and Receptor Specificity of Staphylococcus aureus Binding to Activated Platelets. J. Immunol. 173: 1258-1265 [Abstract] [Full Text]  
  • Pratten, J., Nazhat, S. N., Blaker, J. J., Boccaccini, A. R. (2004). In Vitro Attachment of Staphylococcus Epidermidis to Surgical Sutures with and without Ag-Containing Bioactive Glass Coating. J Biomater Appl 19: 47-57 [Abstract]  
  • Bustanji, Y., Arciola, C. R., Conti, M., Mandello, E., Montanaro, L., Samori, B. (2003). Dynamics of the interaction between a fibronectin molecule and a living bacterium under mechanical force. Proc. Natl. Acad. Sci. USA 100: 13292-13297 [Abstract] [Full Text]  
  • Brouillette, E., Talbot, B. G., Malouin, F. (2003). The Fibronectin-Binding Proteins of Staphylococcus aureus May Promote Mammary Gland Colonization in a Lactating Mouse Model of Mastitis. Infect. Immun. 71: 2292-2295 [Abstract] [Full Text]  
  • Cunnion, K. M., Frank, M. M. (2003). Complement Activation Influences Staphylococcus aureus Adherence to Endothelial Cells. Infect. Immun. 71: 1321-1327 [Abstract] [Full Text]  
  • Petersen, F. C., Assev, S., van der Mei, H. C., Busscher, H. J., Scheie, A. A. (2002). Functional Variation of the Antigen I/II Surface Protein in Streptococcus mutans and Streptococcus intermedius. Infect. Immun. 70: 249-256 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»