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Infection and Immunity, May 2001, p. 3516-3518, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3516-3518.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

YopB of Yersinia enterocolitica Is Essential for YopE Translocation

Roland Nordfelth and Hans Wolf-Watz*

Department of Cell and Molecular Biology, Umeå University, 901 87 Umeå, Sweden

Received 6 November 2000/Returned for modification 8 January 2001/Accepted 19 February 2001

A previous study has shown that YopB of Yersinia spp. is essential for translocation of Yop effectors across the eucaryotic plasma membrane (M.-P. Sory and G. R. Cornelis, Mol. Microbiol. 14:583-594, 1994). However, this role was recently challenged (V. T. Lee and O. Schneewind, Mol. Microbiol. 31:1619-1629, 1999). Using protease protection and digitonin extraction, we reconfirm that YopB of Yersinia enterocolitica is essential for the translocation of YopE into HeLa cell monolayers.

* Corresponding author. Mailing address: Department of Cell and Molecular Biology, Umeå University, 901 87 Umeå, Sweden. Phone: 46 90 785 25 30. E-mail: hans.wolf-watz{at}cmb.umu.se.

Infection and Immunity, May 2001, p. 3516-3518, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.3516-3518.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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