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Infection and Immunity, June 2001, p. 3556-3561, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3556-3561.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Early Local Cytokine Profiles in Strains of Mice with Different Outcomes from Chlamydial Genital Tract Infection

Toni Darville,1,2,* Charles W. Andrews Jr.,3 James D. Sikes,1,2 Patrick L. Fraley,1 and Roger. G. Rank2

Department of Pediatric Infectious Diseases, Arkansas Children's Hospital,1 and Department of Microbiology and Immunology, University of Arkansas for Medical Sciences,2 Little Rock, Arkansas, and Department of Pathology, Sacred Heart Medical Center, Spokane, Washington3

Received 18 October 2000/Returned for modification 10 January 2001/Accepted 13 March 2001

In this study, we expand on the examination of genetically determined differences in host responses that correlate with clearance of Chlamydia trachomatis from the genital tract. We infected C57BL/6, BALB/c, and C3H/HeN mice with the mouse pneumonitis agent of C. trachomatis (MoPn). C57BL/6 mice had the shortest course of infection (22 days) and the lowest incidence of severe hydrosalpinx. BALB/c mice also had a short course of infection (25 days), but all developed hydrosalpinx. C3H/HeN mice had the longest course of infection (38 days), and all developed severe hydrosalpinx. Determination of local cytokine responses by enzyme-linked immunosorbent assay (ELISA) of genital tract secretions revealed that the levels of the proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha ) and interleukin-1beta (IL-1beta ) were significantly increased in the C57BL/6 and BALB/c strains compared to those in the C3H/HeN strain whereas the level of IL-6 was not different. The level of the neutrophil chemokine macrophage inflammatory protein 2 (MIP-2) was increased during the first week of infection in all three strains but was significantly higher in the BALB/c strain, the strain with the most rapid influx of neutrophils into the genital tract. Prolonged detection of MIP-2 in C3H/HeN mice was associated with a protracted presence of neutrophils in the genital tract. Early increases in the levels of the proinflammatory cytokines TNF-alpha and IL-1beta are associated with earlier eradication of infection in the C57BL/6 and BALB/c strains than in the C3H/HeN strain. Increased levels of MIP-2 and neutrophils in BALB/c and C3H/HeN mice relative to C57BL/6 mice suggest that these responses may contribute to pathology.

* Corresponding author. Mailing address: Department of Pediatrics and Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72202. Phone: (501) 686-7430. Fax: (501) 320-3551. E-mail: darvilletonil{at}exchange.uams.edu.

Infection and Immunity, June 2001, p. 3556-3561, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3556-3561.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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