This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Darville, T.
Right arrow Articles by Rank, Roger. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Darville, T.
Right arrow Articles by Rank, Roger. G.

 Previous Article  |  Next Article 

Infection and Immunity, June 2001, p. 3556-3561, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3556-3561.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Early Local Cytokine Profiles in Strains of Mice with Different Outcomes from Chlamydial Genital Tract Infection

Toni Darville,1,2,* Charles W. Andrews Jr.,3 James D. Sikes,1,2 Patrick L. Fraley,1 and Roger. G. Rank2

Department of Pediatric Infectious Diseases, Arkansas Children's Hospital,1 and Department of Microbiology and Immunology, University of Arkansas for Medical Sciences,2 Little Rock, Arkansas, and Department of Pathology, Sacred Heart Medical Center, Spokane, Washington3

Received 18 October 2000/Returned for modification 10 January 2001/Accepted 13 March 2001

In this study, we expand on the examination of genetically determined differences in host responses that correlate with clearance of Chlamydia trachomatis from the genital tract. We infected C57BL/6, BALB/c, and C3H/HeN mice with the mouse pneumonitis agent of C. trachomatis (MoPn). C57BL/6 mice had the shortest course of infection (22 days) and the lowest incidence of severe hydrosalpinx. BALB/c mice also had a short course of infection (25 days), but all developed hydrosalpinx. C3H/HeN mice had the longest course of infection (38 days), and all developed severe hydrosalpinx. Determination of local cytokine responses by enzyme-linked immunosorbent assay (ELISA) of genital tract secretions revealed that the levels of the proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha ) and interleukin-1beta (IL-1beta ) were significantly increased in the C57BL/6 and BALB/c strains compared to those in the C3H/HeN strain whereas the level of IL-6 was not different. The level of the neutrophil chemokine macrophage inflammatory protein 2 (MIP-2) was increased during the first week of infection in all three strains but was significantly higher in the BALB/c strain, the strain with the most rapid influx of neutrophils into the genital tract. Prolonged detection of MIP-2 in C3H/HeN mice was associated with a protracted presence of neutrophils in the genital tract. Early increases in the levels of the proinflammatory cytokines TNF-alpha and IL-1beta are associated with earlier eradication of infection in the C57BL/6 and BALB/c strains than in the C3H/HeN strain. Increased levels of MIP-2 and neutrophils in BALB/c and C3H/HeN mice relative to C57BL/6 mice suggest that these responses may contribute to pathology.

* Corresponding author. Mailing address: Department of Pediatrics and Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72202. Phone: (501) 686-7430. Fax: (501) 320-3551. E-mail: darvilletonil{at}exchange.uams.edu.

Infection and Immunity, June 2001, p. 3556-3561, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3556-3561.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zhou, X., Chen, Q., Moore, J., Kolls, J. K., Halperin, S., Wang, J. (2009). Critical Role of the Interleukin-17/Interleukin-17 Receptor Axis in Regulating Host Susceptibility to Respiratory Infection with Chlamydia Species. Infect. Immun. 77: 5059-5070 [Abstract] [Full Text]  
  • Bai, H., Cheng, J., Gao, X., Joyee, A. G., Fan, Y., Wang, S., Jiao, L., Yao, Z., Yang, X. (2009). IL-17/Th17 Promotes Type 1 T Cell Immunity against Pulmonary Intracellular Bacterial Infection through Modulating Dendritic Cell Function. J. Immunol. 183: 5886-5895 [Abstract] [Full Text]  
  • Jayarapu, K., Kerr, M. S., Katschke, A., Johnson, R. M. (2009). Chlamydia muridarum-Specific CD4 T-Cell Clones Recognize Infected Reproductive Tract Epithelial Cells in an Interferon-Dependent Fashion. Infect. Immun. 77: 4469-4479 [Abstract] [Full Text]  
  • Abdul-Sater, A. A., Koo, E., Hacker, G., Ojcius, D. M. (2009). Inflammasome-dependent Caspase-1 Activation in Cervical Epithelial Cells Stimulates Growth of the Intracellular Pathogen Chlamydia trachomatis. J. Biol. Chem. 284: 26789-26796 [Abstract] [Full Text]  
  • Nagarajan, U. M., Prantner, D., Sikes, J. D., Andrews, C. W. Jr., Goodwin, A. M., Nagarajan, S., Darville, T. (2008). Type I Interferon Signaling Exacerbates Chlamydia muridarum Genital Infection in a Murine Model. Infect. Immun. 76: 4642-4648 [Abstract] [Full Text]  
  • Bas, S., Neff, L., Vuillet, M., Spenato, U., Seya, T., Matsumoto, M., Gabay, C. (2008). The Proinflammatory Cytokine Response to Chlamydia trachomatis Elementary Bodies in Human Macrophages Is Partly Mediated by a Lipoprotein, the Macrophage Infectivity Potentiator, through TLR2/TLR1/TLR6 and CD14. J. Immunol. 180: 1158-1168 [Abstract] [Full Text]  
  • Darville, T., Welter-Stahl, L., Cruz, C., Sater, A. A., Andrews, C. W. Jr., Ojcius, D. M. (2007). Effect of the Purinergic Receptor P2X7 on Chlamydia Infection in Cervical Epithelial Cells and Vaginally Infected Mice. J. Immunol. 179: 3707-3714 [Abstract] [Full Text]  
  • Miyairi, I., Tatireddigari, V. R. R. A., Mahdi, O. S., Rose, L. A., Belland, R. J., Lu, L., Williams, R. W., Byrne, G. I. (2007). The p47 GTPases Iigp2 and Irgb10 Regulate Innate Immunity and Inflammation to Murine Chlamydia psittaci Infection. J. Immunol. 179: 1814-1824 [Abstract] [Full Text]  
  • Murthy, A. K., Chambers, J. P., Meier, P. A., Zhong, G., Arulanandam, B. P. (2007). Intranasal Vaccination with a Secreted Chlamydial Protein Enhances Resolution of Genital Chlamydia muridarum Infection, Protects against Oviduct Pathology, and Is Highly Dependent upon Endogenous Gamma Interferon Production. Infect. Immun. 75: 666-676 [Abstract] [Full Text]  
  • Lim, J. K., Lu, W., Hartley, O., DeVico, A. L. (2006). N-terminal proteolytic processing by cathepsin G converts RANTES/CCL5 and related analogs into a truncated 4-68 variant. J. Leukoc. Biol. 80: 1395-1404 [Abstract] [Full Text]  
  • Ramsey, K.H., Sigar, I.M., Schripsema, J. H., Shaba, N., Cohoon, K. P. (2005). Expression of Matrix Metalloproteinases Subsequent to Urogenital Chlamydia muridarum Infection of Mice. Infect. Immun. 73: 6962-6973 [Abstract] [Full Text]  
  • Bilenki, L., Wang, S., Yang, J., Fan, Y., Joyee, A. G., Yang, X. (2005). NK T Cell Activation Promotes Chlamydia trachomatis Infection In Vivo. J. Immunol. 175: 3197-3206 [Abstract] [Full Text]  
  • Maxion, H. K., Liu, W., Chang, M.-H., Kelly, K. A. (2004). The Infecting Dose of Chlamydia muridarum Modulates the Innate Immune Response and Ascending Infection. Infect. Immun. 72: 6330-6340 [Abstract] [Full Text]  
  • Johnson, R. M. (2004). Murine Oviduct Epithelial Cell Cytokine Responses to Chlamydia muridarum Infection Include Interleukin-12-p70 Secretion. Infect. Immun. 72: 3951-3960 [Abstract] [Full Text]  
  • Darville, T., O'Neill, J. M., Andrews, C. W. Jr., Nagarajan, U. M., Stahl, L., Ojcius, D. M. (2003). Toll-Like Receptor-2, but Not Toll-Like Receptor-4, Is Essential for Development of Oviduct Pathology in Chlamydial Genital Tract Infection. J. Immunol. 171: 6187-6197 [Abstract] [Full Text]  
  • Perfettini, J.-L., Ojcius, D. M., Andrews, C. W. Jr., Korsmeyer, S. J., Rank, R. G., Darville, T. (2003). Role of Proapoptotic BAX in Propagation of Chlamydia muridarum (the Mouse Pneumonitis Strain of Chlamydia trachomatis) and the Host Inflammatory Response. J. Biol. Chem. 278: 9496-9502 [Abstract] [Full Text]  
  • Morrison, R. P., Caldwell, H. D. (2002). Immunity to Murine Chlamydial Genital Infection. Infect. Immun. 70: 2741-2751 [Full Text]  
  • Perfettini, J.-L., Darville, T., Dautry-Varsat, A., Rank, R. G., Ojcius, D. M. (2002). Inhibition of Apoptosis by Gamma Interferon in Cells and Mice Infected with Chlamydia muridarum (the Mouse Pneumonitis Strain of Chlamydia trachomatis). Infect. Immun. 70: 2559-2565 [Abstract] [Full Text]  
  • Ramsey, K. H., Sigar, I. M., Rana, S. V., Gupta, J., Holland, S. M., Byrne, G. I. (2001). Role for Inducible Nitric Oxide Synthase in Protection from Chronic Chlamydia trachomatis Urogenital Disease in Mice and Its Regulation by Oxygen Free Radicals. Infect. Immun. 69: 7374-7379 [Abstract] [Full Text]  
  • Darville, T., Andrews, C. W. Jr., Sikes, J. D., Fraley, P. L., Braswell, L., Rank, R. G. (2001). Mouse Strain-Dependent Chemokine Regulation of the Genital Tract T Helper Cell Type 1 Immune Response. Infect. Immun. 69: 7419-7424 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»