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Infection and Immunity, June 2001, p. 3663-3669, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3663-3669.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Lipopolysaccharide of Burkholderia cepacia and Its Unique Character To Stimulate Murine Macrophages with Relative Lack of Interleukin-1beta -Inducing Ability

Hirofumi Shimomura,1 Motohiro Matsuura,1,* Shinji Saito,1 Yoshikazu Hirai,1 Yasunori Isshiki,2 and Kazuyoshi Kawahara2

Department of Microbiology, Jichi Medical School, Tochigi 329-0498,1 and Department of Bacteriology, The Kitasato Institute, Tokyo 108-8642,2 Japan

Received 27 December 2000/Returned for modification 9 February 2001/Accepted 5 March 2001

Lipopolysaccharide (LPS) of Burkholderia cepacia was purified by the conventional phenol-water extraction method (preparation BcLPS-1), followed by enzymatic treatments with DNase, RNase, trypsin, and proteinase K (preparation BcLPS-2), and finally by deoxycholate-phenol-water extraction (preparation BcLPS-3). Cells of LPS-hyporesponsive C3H/HeJ mice were activated by both the BcLPS-1 and the BcLPS-2 preparations but barely activated by BcLPS-3. When LPS-responsive C3H/HeN mice were used as targets, endotoxic activities such as lethal toxicity to galactosamine-sensitized mice, mitogenicity to spleen cells, and activation of macrophages to induce tumor necrosis factor alpha and interleukin-6 (IL-6) were strongly exhibited even by highly purified BcLPS-3 at levels comparable to those of the highly active enterobacterial LPS of Salmonella enterica serovar Abortus-equi (SaeLPS), used as the control. The ability of BcLPS-3 to activate murine macrophages for induction of IL-1beta was, however, much weaker than that of SaeLPS. Both accumulation of pro-IL-1beta protein and expression of IL-1beta mRNA in macrophages by stimulation with BcLPS-3 were much weaker than by stimulation with SaeLPS. These results indicate that LPS of B. cepacia has the potential to play a role as a pathogenic factor with strong activity comparable to that of usual enterobacterial LPS, but unlike the latter, this LPS has a relative lack of ability in the activation of murine macrophages to induce IL-1beta . The lack of IL-1beta -inducing ability appears to be caused by incomplete signal transduction somewhere in the upstream step(s) of IL-1beta gene transcription.

* Corresponding author. Mailing address: Department of Microbiology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Tochigi 329-0498, Japan. Phone: 81-285-58-7332. Fax: 81-285-44-1175. E-mail: mmatsuur{at}jichi.ac.jp.

Infection and Immunity, June 2001, p. 3663-3669, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3663-3669.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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