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Infection and Immunity, June 2001, p. 3670-3677, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3670-3677.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Analysis of Mechanisms Associated with Loss of Infectivity of Clonal Populations of Borrelia burgdorferi B31MI

John V. McDowell,1 Shian Ying Sung,1 Maria Labandeira-Rey,2 Jon T. Skare,2 and Richard T. Marconi1,*

Department of Microbiology and Immunology, School of Medicine, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298-0678,1 and Department of Microbiology and Immunology, The Texas A & M University System Health Science Center, College Station, Texas 77843-11142

Received 21 December 2000/Accepted 7 March 2001

Numerous studies have provided suggestive evidence that the loss of plasmids correlates with the loss of infectivity of the Lyme disease spirochetes. In this study we have further investigated this correlation. Clonal populations were obtained from the skin of a mouse infected for 3 months with a clonal population of Borrelia burgdorferi B31MI. The complete plasmid compositions of these populations were determined using a combination of PCR and Southern hybridization. The infectivities of clones differing in plasmid composition were tested using the C3H-HeJ murine model for Lyme disease. While several clones were found to be noninfectious, a correlation between the loss of a specific plasmid and loss of infectivity in the clones analyzed in this report was not observed. While it is clear from recent studies that the loss of some specific plasmids results in attenuated virulence, this study demonstrates that additional mechanisms also contribute to the loss of infectivity.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, School of Medicine, Medical College of Virginia at Virginia Commonwealth University, Richmond, VA 23298-0678. Phone: (804) 828-3779. Fax: (804) 828-9946. E-mail: rmarconi{at}hsc.vcu.edu.

Infection and Immunity, June 2001, p. 3670-3677, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3670-3677.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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