This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Magee, A. D.
Right arrow Articles by Yother, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Magee, A. D.
Right arrow Articles by Yother, J.

 Previous Article  |  Next Article 

Infection and Immunity, June 2001, p. 3755-3761, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3755-3761.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Requirement for Capsule in Colonization by Streptococcus pneumoniae

Ashalla D. Magee and Janet Yother*

Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 2 February 2001/Returned for modification 2 March 2001/Accepted 20 March 2001

Nasopharyngeal colonization is a necessary first step in the pathogenesis of Streptococcus pneumoniae. Using isolates containing defined mutations in the S. pneumoniae capsule locus, we found that expression of the capsular polysaccharide is essential for colonization by the type 2 strain D39 and the type 3 strains A66 and WU2. Nonencapsulated derivatives of each of these strains were unable to colonize BALB/cByJ mice. Similarly, type 3 mutants that produced <6% of the parental amounts of capsule could not colonize. Capsule production equivalent to that of the parent strain was not required for efficient colonization, however, as type 3 mutants producing approximately 20% of the parental amounts of capsule colonized as effectively as the parent. This 80% reduction in capsule level had only a minimal effect on intraperitoneal virulence but caused a significant reduction in virulence via the intravenous route. In the X-linked immunodeficient CBA/N mouse, the type 3 mutant producing ~20% of the parental amount of capsule (AM188) was diminished in its ability to cause invasive disease and death following intranasal inoculation. Following intravenous or intraperitoneal challenge, however, only extended survival times were observed. Our results demonstrate an additional role for capsule in the pathogenesis of S. pneumoniae and show that isolates producing reduced levels of capsule can remain highly virulent.

* Corresponding author. Mailing address: Department of Microbiology, BBRB 661/12, 845 19th St. South, Birmingham, AL 35294. Phone: (205) 934-9531. Fax: (205) 975-6715. E-mail: jyother{at}uab.edu.

Infection and Immunity, June 2001, p. 3755-3761, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3755-3761.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Noske, N., Kammerer, U., Rohde, M., Hammerschmidt, S. (2009). Pneumococcal Interaction with Human Dendritic Cells: Phagocytosis, Survival, and Induced Adaptive Immune Response Are Manipulated by PavA. J. Immunol. 183: 1952-1963 [Abstract] [Full Text]  
  • Cron, L. E., Bootsma, H. J., Noske, N., Burghout, P., Hammerschmidt, S., Hermans, P. W. M. (2009). Surface-associated lipoprotein PpmA of Streptococcus pneumoniae is involved in colonization in a strain-specific manner. Microbiology 155: 2401-2410 [Abstract] [Full Text]  
  • Lebeer, S., Verhoeven, T. L. A., Francius, G., Schoofs, G., Lambrichts, I., Dufrene, Y., Vanderleyden, J., De Keersmaecker, S. C. J. (2009). Identification of a Gene Cluster for the Biosynthesis of a Long, Galactose-Rich Exopolysaccharide in Lactobacillus rhamnosus GG and Functional Analysis of the Priming Glycosyltransferase. Appl. Environ. Microbiol. 75: 3554-3563 [Abstract] [Full Text]  
  • Moscoso, M., Garcia, E. (2009). Transcriptional Regulation of the Capsular Polysaccharide Biosynthesis Locus of Streptococcus Pneumoniae: a Bioinformatic Analysis. DNA Res 16: 177-186 [Abstract] [Full Text]  
  • Forsee, W. T., Cartee, R. T., Yother, J. (2009). A Kinetic Model for Chain Length Modulation of Streptococcus pneumoniae Cellubiuronan Capsular Polysaccharide by Nucleotide Sugar Donor Concentrations. J. Biol. Chem. 284: 11836-11844 [Abstract] [Full Text]  
  • Sabharwal, V., Ram, S., Figueira, M., Park, I. H., Pelton, S. I. (2009). Role of Complement in Host Defense against Pneumococcal Otitis Media. Infect. Immun. 77: 1121-1127 [Abstract] [Full Text]  
  • Attali, C., Durmort, C., Vernet, T., Di Guilmi, A. M. (2008). The Interaction of Streptococcus pneumoniae with Plasmin Mediates Transmigration across Endothelial and Epithelial Monolayers by Intercellular Junction Cleavage. Infect. Immun. 76: 5350-5356 [Abstract] [Full Text]  
  • Munoz-Elias, E. J., Marcano, J., Camilli, A. (2008). Isolation of Streptococcus pneumoniae Biofilm Mutants and Their Characterization during Nasopharyngeal Colonization. Infect. Immun. 76: 5049-5061 [Abstract] [Full Text]  
  • Mahdi, L. K., Ogunniyi, A. D., LeMessurier, K. S., Paton, J. C. (2008). Pneumococcal Virulence Gene Expression and Host Cytokine Profiles during Pathogenesis of Invasive Disease. Infect. Immun. 76: 646-657 [Abstract] [Full Text]  
  • Bootsma, H. J., Egmont-Petersen, M., Hermans, P. W. M. (2007). Analysis of the In Vitro Transcriptional Response of Human Pharyngeal Epithelial Cells to Adherent Streptococcus pneumoniae: Evidence for a Distinct Response to Encapsulated Strains. Infect. Immun. 75: 5489-5499 [Abstract] [Full Text]  
  • Mills, M. F., Marquart, M. E., McDaniel, L. S. (2007). Localization of PcsB of Streptococcus pneumoniae and Its Differential Expression in Response to Stress. J. Bacteriol. 189: 4544-4546 [Abstract] [Full Text]  
  • Xayarath, B., Yother, J. (2007). Mutations Blocking Side Chain Assembly, Polymerization, or Transport of a Wzy-Dependent Streptococcus pneumoniae Capsule Are Lethal in the Absence of Suppressor Mutations and Can Affect Polymer Transfer to the Cell Wall. J. Bacteriol. 189: 3369-3381 [Abstract] [Full Text]  
  • Ogunniyi, A. D., LeMessurier, K. S., Graham, R. M. A., Watt, J. M., Briles, D. E., Stroeher, U. H., Paton, J. C. (2007). Contributions of Pneumolysin, Pneumococcal Surface Protein A (PspA), and PspC to Pathogenicity of Streptococcus pneumoniae D39 in a Mouse Model. Infect. Immun. 75: 1843-1851 [Abstract] [Full Text]  
  • Allegrucci, M., Sauer, K. (2007). Characterization of Colony Morphology Variants Isolated from Streptococcus pneumoniae Biofilms. J. Bacteriol. 189: 2030-2038 [Abstract] [Full Text]  
  • Lowe, B. A., Miller, J. D., Neely, M. N. (2007). Analysis of the Polysaccharide Capsule of the Systemic Pathogen Streptococcus iniae and Its Implications in Virulence. Infect. Immun. 75: 1255-1264 [Abstract] [Full Text]  
  • Lanie, J. A., Ng, W.-L., Kazmierczak, K. M., Andrzejewski, T. M., Davidsen, T. M., Wayne, K. J., Tettelin, H., Glass, J. I., Winkler, M. E. (2007). Genome Sequence of Avery's Virulent Serotype 2 Strain D39 of Streptococcus pneumoniae and Comparison with That of Unencapsulated Laboratory Strain R6. J. Bacteriol. 189: 38-51 [Abstract] [Full Text]  
  • McEllistrem, M. C., Ransford, J. V., Khan, S. A. (2007). Characterization of In Vitro Biofilm-Associated Pneumococcal Phase Variants of a Clinically Relevant Serotype 3 Clone. J. Clin. Microbiol. 45: 97-101 [Abstract] [Full Text]  
  • Nelson, A. L., Roche, A. M., Gould, J. M., Chim, K., Ratner, A. J., Weiser, J. N. (2007). Capsule Enhances Pneumococcal Colonization by Limiting Mucus-Mediated Clearance. Infect. Immun. 75: 83-90 [Abstract] [Full Text]  
  • Loeffler, J. M., Fischetti, V. A. (2006). Lysogeny of Streptococcus pneumoniae with MM1 Phage: Improved Adherence and Other Phenotypic Changes.. Infect. Immun. 74: 4486-4495 [Abstract] [Full Text]  
  • Pettigrew, M. M., Fennie, K. P., York, M. P., Daniels, J., Ghaffar, F. (2006). Variation in the Presence of Neuraminidase Genes among Streptococcus pneumoniae Isolates with Identical Sequence Types.. Infect. Immun. 74: 3360-3365 [Abstract] [Full Text]  
  • Morona, J. K., Morona, R., Paton, J. C. (2006). Attachment of capsular polysaccharide to the cell wall of Streptococcus pneumoniae type 2 is required for invasive disease. Proc. Natl. Acad. Sci. USA 103: 8505-8510 [Abstract] [Full Text]  
  • Allegrucci, M., Hu, F. Z., Shen, K., Hayes, J., Ehrlich, G. D., Post, J. C., Sauer, K. (2006). Phenotypic Characterization of Streptococcus pneumoniae Biofilm Development.. J. Bacteriol. 188: 2325-2335 [Abstract] [Full Text]  
  • Cartee, R. T., Forsee, W. T., Bender, M. H., Ambrose, K. D., Yother, J. (2005). CpsE from Type 2 Streptococcus pneumoniae Catalyzes the Reversible Addition of Glucose-1-Phosphate to a Polyprenyl Phosphate Acceptor, Initiating Type 2 Capsule Repeat Unit Formation. J. Bacteriol. 187: 7425-7433 [Abstract] [Full Text]  
  • Briles, D. E., Novak, L., Hotomi, M., van Ginkel, F. W., King, J. (2005). Nasal Colonization with Streptococcus pneumoniae Includes Subpopulations of Surface and Invasive Pneumococci. Infect. Immun. 73: 6945-6951 [Abstract] [Full Text]  
  • Hammerschmidt, S., Wolff, S., Hocke, A., Rosseau, S., Muller, E., Rohde, M. (2005). Illustration of Pneumococcal Polysaccharide Capsule during Adherence and Invasion of Epithelial Cells. Infect. Immun. 73: 4653-4667 [Abstract] [Full Text]  
  • Cartee, R. T., Forsee, W. T., Yother, J. (2005). Initiation and Synthesis of the Streptococcus pneumoniae Type 3 Capsule on a Phosphatidylglycerol Membrane Anchor. J. Bacteriol. 187: 4470-4479 [Abstract] [Full Text]  
  • Miller, J. D., Neely, M. N. (2005). Large-Scale Screen Highlights the Importance of Capsule for Virulence in the Zoonotic Pathogen Streptococcus iniae. Infect. Immun. 73: 921-934 [Abstract] [Full Text]  
  • Reed, J. M., O'Callaghan, R. J., Girgis, D. O., McCormick, C. C., Caballero, A. R., Marquart, M. E. (2005). Ocular Virulence of Capsule-Deficient Streptococcus pneumoniae in a Rabbit Keratitis Model. IOVS 46: 604-608 [Abstract] [Full Text]  
  • Jefferies, J. M. C., Smith, A., Clarke, S. C., Dowson, C., Mitchell, T. J. (2004). Genetic Analysis of Diverse Disease-Causing Pneumococci Indicates High Levels of Diversity within Serotypes and Capsule Switching. J. Clin. Microbiol. 42: 5681-5688 [Abstract] [Full Text]  
  • McCool, T. L., Weiser, J. N. (2004). Limited Role of Antibody in Clearance of Streptococcus pneumoniae in a Murine Model of Colonization. Infect. Immun. 72: 5807-5813 [Abstract] [Full Text]  
  • Donlan, R. M., Piede, J. A., Heyes, C. D., Sanii, L., Murga, R., Edmonds, P., El-Sayed, I., El-Sayed, M. A. (2004). Model System for Growing and Quantifying Streptococcus pneumoniae Biofilms In Situ and in Real Time. Appl. Environ. Microbiol. 70: 4980-4988 [Abstract] [Full Text]  
  • van Ginkel, F. W., McGhee, J. R., Watt, J. M., Campos-Torres, A., Parish, L. A., Briles, D. E. (2003). Pneumococcal carriage results in ganglioside-mediated olfactory tissue infection. Proc. Natl. Acad. Sci. USA 100: 14363-14367 [Abstract] [Full Text]  
  • Bender, M. H., Cartee, R. T., Yother, J. (2003). Positive Correlation between Tyrosine Phosphorylation of CpsD and Capsular Polysaccharide Production in Streptococcus pneumoniae. J. Bacteriol. 185: 6057-6066 [Abstract] [Full Text]  
  • Waite, R. D., Penfold, D. W., Struthers, J. K., Dowson, C. G. (2003). Spontaneous sequence duplications within capsule genes cap8E and tts control phase variation in Streptococcus pneumoniae serotypes 8 and 37. Microbiology 149: 497-504 [Abstract] [Full Text]  
  • Balachandran, P., Brooks-Walter, A., Virolainen-Julkunen, A., Hollingshead, S. K., Briles, D. E. (2002). Role of Pneumococcal Surface Protein C in Nasopharyngeal Carriage and Pneumonia and Its Ability To Elicit Protection against Carriage of Streptococcus pneumoniae. Infect. Immun. 70: 2526-2534 [Abstract] [Full Text]  
  • Bender, M. H., Yother, J. (2001). CpsB Is a Modulator of Capsule-associated Tyrosine Kinase Activity in Streptococcus pneumoniae. J. Biol. Chem. 276: 47966-47974 [Abstract] [Full Text]  
  • Orihuela, C. J., Mills, J., Robb, C. W., Wilson, C. J., Watson, D. A., Niesel, D. W. (2001). Streptococcus pneumoniae PstS Production Is Phosphate Responsive and Enhanced during Growth in the Murine Peritoneal Cavity. Infect. Immun. 69: 7565-7571 [Abstract] [Full Text]  
  • Cartee, R. T., Forsee, W. T., Jensen, J. W., Yother, J. (2001). Expression of the Streptococcus pneumoniae Type 3 Synthase in Escherichia coli. ASSEMBLY OF TYPE 3 POLYSACCHARIDE ON A LIPID PRIMER. J. Biol. Chem. 276: 48831-48839 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»