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Infection and Immunity, June 2001, p. 3916-3923, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3916-3923.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

LYT1 Protein Is Required for Efficient In Vitro Infection by Trypanosoma cruzi

Rebeca Manning-Cela,1,2 Arantxa Cortés,3 Elena González-Rey,3 Wesley C. Van Voorhis,4 John Swindle,1,5,* and Antonio González3

Seattle Biomedical Research Institute,1 Departments of Pathobiology and Medicine, University of Washington,4 and Infection Disease Research Institute,5 Seattle, Washington; Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Mexico City, D.F., Mexico2; and Instituto de Parasitología y Biomedicina, CSIC, Granada, Spain3

Received 11 January 2001/Returned for modification 28 February 2001/Accepted 22 March 2001

Trypanosoma cruzi invasion of host cells involves several discrete steps: attachment, parasite internalization mediated by recruitment and fusion of host cell lysosomes, and escape from the parasitophorous vacuole to liberate amastigotes to multiply freely in the cytosol. This report describes the initial characterization of the LYT1 gene and the demonstration that the gene product is involved in cell lysis and infectivity. Mutational analysis demonstrated that deletion of LYT1 resulted in attenuation of infection, which was associated with diminished hemolytic activity. Reintroduction of LYT1 restored infectivity in null mutants, confirming the critical role of LYT1 in infection. Additionally, in vitro stage transition experiments with LYT1-deficient lines showed that these parasites converted to extracellular amastigote-like cells and metacyclic trypomastigotes more rapidly than wild-type parasites, suggesting that the diminished infectivity was not a result of the LYT1 deficiency that affected the parasite's ability to complete the life cycle.

* Corresponding author. Mailing address: Infection Disease Research Institute, 1124 Columbia St., Suite 600, Seattle, WA 98104. Phone: (206)381-0883. Fax: (206) 381-3678. E-mail: jswindle{at}idri.org.

Infection and Immunity, June 2001, p. 3916-3923, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.3916-3923.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zago, M. P., Barrio, A. B., Cardozo, R. M., Duffy, T., Schijman, A. G., Basombrio, M. A. (2008). Impairment of Infectivity and Immunoprotective Effect of a LYT1 Null Mutant of Trypanosoma cruzi. Infect. Immun. 76: 443-451 [Abstract] [Full Text]  
  • Manning-Cela, R., Gonzalez, A., Swindle, J. (2002). Alternative Splicing of LYT1 Transcripts in Trypanosoma cruzi. Infect. Immun. 70: 4726-4728 [Abstract] [Full Text]  


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