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Infection and Immunity, June 2001, p. 4027-4033, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.4027-4033.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

EspG, a Novel Type III System-Secreted Protein from Enteropathogenic Escherichia coli with Similarities to VirA of Shigella flexneri

Simon J. Elliott,1,dagger Efrosinia O. Krejany,2 Jay L. Mellies,3 Roy M. Robins-Browne,2 Chihiro Sasakawa,4 and James B. Kaper1,*

Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 212011; Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria 3052, Australia2; Department of Biology, Reed College, Portland, Oregon 972023; and Division of Bacterial Infection, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan4

Received 18 August 2000/Returned for modification 30 October 2000/Accepted 12 March 2001

The function of the rorf2 gene located on the locus of enterocyte effacement (LEE) pathogenicity island of enteropathogenic Escherichia coli (EPEC) has not been described. We report that rorf2 encodes a novel protein, named EspG, which is secreted by the type III secretory system and which is translocated into host epithelial cells. EspG is homologous with Shigella flexneri protein VirA, and the cloned espG (rorf2) gene can rescue invasion in a Shigella virA mutant, indicating that these proteins are functionally equivalent in Shigella. An EPEC espG mutant had no apparent defects in in vitro assays of virulence phenotypes, but a rabbit diarrheagenic E. coli strain carrying a mutant espG showed diminished intestinal colonization and yet diarrheal attack rates similar to those of the wild type. A second EspG homolog, Orf3, is encoded on the EspC pathogenicity islet. The cloned orf3 gene could also rescue invasion in a Shigella virA mutant, but an EPEC espG orf3 double mutant was not diminished in any tested in vitro assays for EPEC virulence factors. Our results indicate that EspG plays an accessory but as yet undefined role in EPEC virulence that may involve intestinal colonization.

* Corresponding author. Mailing address: Center for Vaccine Development, University of Maryland School of Medicine, 685 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706-2344. Fax: (410) 706-0182. E-mail: jkaper{at}umaryland.edu.

dagger Present address: Department of Pediatric Infectious Diseases, Johns Hopkins School of Medicine, Baltimore, MD 21205.

Infection and Immunity, June 2001, p. 4027-4033, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.4027-4033.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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