Previous Article | Next Article 
Infection and Immunity, June 2001, p. 4027-4033, Vol. 69, No. 6
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.6.4027-4033.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
EspG, a Novel Type III System-Secreted Protein from
Enteropathogenic Escherichia coli with Similarities to
VirA of Shigella flexneri
Simon J.
Elliott,1,
Efrosinia O.
Krejany,2
Jay L.
Mellies,3
Roy M.
Robins-Browne,2
Chihiro
Sasakawa,4 and
James B.
Kaper1,*
Center for Vaccine Development and Department of
Microbiology and Immunology, University of Maryland School of Medicine,
Baltimore, Maryland 212011; Department
of Microbiology and Immunology, The University of Melbourne,
Parkville, Victoria 3052, Australia2;
Department of Biology, Reed College, Portland, Oregon
972023; and Division of Bacterial
Infection, Department of Microbiology and Immunology, Institute of
Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan4
Received 18 August 2000/Returned for modification 30 October
2000/Accepted 12 March 2001
The function of the rorf2 gene located on the locus
of enterocyte effacement (LEE) pathogenicity island of enteropathogenic Escherichia coli (EPEC) has not been described. We
report that rorf2 encodes a novel protein, named EspG,
which is secreted by the type III secretory system and which is
translocated into host epithelial cells. EspG is homologous with
Shigella flexneri protein VirA, and the cloned
espG (rorf2) gene can rescue invasion in a Shigella virA mutant, indicating that these proteins
are functionally equivalent in Shigella. An EPEC
espG mutant had no apparent defects in in vitro assays
of virulence phenotypes, but a rabbit diarrheagenic E.
coli strain carrying a mutant espG showed
diminished intestinal colonization and yet diarrheal attack rates
similar to those of the wild type. A second EspG homolog, Orf3,
is encoded on the EspC pathogenicity islet. The cloned
orf3 gene could also rescue invasion in a
Shigella virA mutant, but an EPEC espG
orf3 double mutant was not diminished in any tested in vitro
assays for EPEC virulence factors. Our results indicate that EspG plays
an accessory but as yet undefined role in EPEC virulence that may
involve intestinal colonization.
*
Corresponding author. Mailing address: Center for
Vaccine Development, University of Maryland School of Medicine, 685 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706-2344. Fax: (410)
706-0182. E-mail: jkaper{at}umaryland.edu.

Present address: Department of Pediatric Infectious Diseases, Johns
Hopkins School of Medicine, Baltimore, MD
21205.
Infection and Immunity, June 2001, p. 4027-4033, Vol. 69, No. 6
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.6.4027-4033.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Su, M. S.-W., Kao, H.-C., Lin, C.-N., Syu, W.-J.
(2008). Gene l0017 encodes a second chaperone for EspA of enterohaemorrhagic Escherichia coli O157 : H7. Microbiology
154: 1094-1103
[Abstract]
[Full Text]
-
Zurawski, D. V., Mumy, K. L., Badea, L., Prentice, J. A., Hartland, E. L., McCormick, B. A., Maurelli, A. T.
(2008). The NleE/OspZ Family of Effector Proteins Is Required for Polymorphonuclear Transepithelial Migration, a Characteristic Shared by Enteropathogenic Escherichia coli and Shigella flexneri Infections. Infect. Immun.
76: 369-379
[Abstract]
[Full Text]
-
Sharp, F. C., Sperandio, V.
(2007). QseA Directly Activates Transcription of LEE1 in Enterohemorrhagic Escherichia coli. Infect. Immun.
75: 2432-2440
[Abstract]
[Full Text]
-
Hodges, K., Gill, R., Ramaswamy, K., Dudeja, P. K., Hecht, G.
(2006). Rapid activation of Na+/H+ exchange by EPEC is PKC mediated. Am. J. Physiol. Gastrointest. Liver Physiol.
291: G959-G968
[Abstract]
[Full Text]
-
Tobe, T., Beatson, S. A., Taniguchi, H., Abe, H., Bailey, C. M., Fivian, A., Younis, R., Matthews, S., Marches, O., Frankel, G., Hayashi, T., Pallen, M. J.
(2006). An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination. Proc. Natl. Acad. Sci. USA
103: 14941-14946
[Abstract]
[Full Text]
-
Vidal, J. E., Navarro-Garcia, F.
(2006). Efficient Translocation of EspC into Epithelial Cells Depends on Enteropathogenic Escherichia coli and Host Cell Contact. Infect. Immun.
74: 2293-2303
[Abstract]
[Full Text]
-
Kelly, M., Hart, E., Mundy, R., Marches, O., Wiles, S., Badea, L., Luck, S., Tauschek, M., Frankel, G., Robins-Browne, R. M., Hartland, E. L.
(2006). Essential Role of the Type III Secretion System Effector NleB in Colonization of Mice by Citrobacter rodentium. Infect. Immun.
74: 2328-2337
[Abstract]
[Full Text]
-
Sharma, R., Tesfay, S., Tomson, F. L., Kanteti, R. P., Viswanathan, V. K., Hecht, G.
(2006). Balance of bacterial pro- and anti-inflammatory mediators dictates net effect of enteropathogenic Escherichia coli on intestinal epithelial cells. Am. J. Physiol. Gastrointest. Liver Physiol.
290: G685-G694
[Abstract]
[Full Text]
-
Kuwae, A., Matsuzawa, T., Ishikawa, N., Abe, H., Nonaka, T., Fukuda, H., Imajoh-Ohmi, S., Abe, A.
(2006). BopC Is a Novel Type III Effector Secreted by Bordetella bronchiseptica and Has a Critical Role in Type III-dependent Necrotic Cell Death. J. Biol. Chem.
281: 6589-6600
[Abstract]
[Full Text]
-
Borthakur, A., Gill, R. K., Hodges, K., Ramaswamy, K., Hecht, G., Dudeja, P. K.
(2006). Enteropathogenic Escherichia coli inhibits butyrate uptake in Caco-2 cells by altering the apical membrane MCT1 level. Am. J. Physiol. Gastrointest. Liver Physiol.
290: G30-G35
[Abstract]
[Full Text]
-
Marches, O., Wiles, S., Dziva, F., La Ragione, R. M., Schuller, S., Best, A., Phillips, A. D., Hartland, E. L., Woodward, M. J., Stevens, M. P., Frankel, G.
(2005). Characterization of Two Non-Locus of Enterocyte Effacement-Encoded Type III-Translocated Effectors, NleC and NleD, in Attaching and Effacing Pathogens. Infect. Immun.
73: 8411-8417
[Abstract]
[Full Text]
-
Matsuzawa, T., Kuwae, A., Abe, A.
(2005). Enteropathogenic Escherichia coli Type III Effectors EspG and EspG2 Alter Epithelial Paracellular Permeability. Infect. Immun.
73: 6283-6289
[Abstract]
[Full Text]
-
Rodriguez-Escudero, I., Hardwidge, P. R., Nombela, C., Cid, V. J., Finlay, B. B., Molina, M.
(2005). Enteropathogenic Escherichia coli type III effectors alter cytoskeletal function and signalling in Saccharomyces cerevisiae. Microbiology
151: 2933-2945
[Abstract]
[Full Text]
-
Naylor, S. W., Roe, A. J., Nart, P., Spears, K., Smith, David. G. E., Low, J. C., Gally, D. L.
(2005). Escherichia coli O157 : H7 forms attaching and effacing lesions at the terminal rectum of cattle and colonization requires the LEE4 operon. Microbiology
151: 2773-2781
[Abstract]
[Full Text]
-
Kanack, K. J., Crawford, J. A., Tatsuno, I., Karmali, M. A., Kaper, J. B.
(2005). SepZ/EspZ Is Secreted and Translocated into HeLa Cells by the Enteropathogenic Escherichia coli Type III Secretion System. Infect. Immun.
73: 4327-4337
[Abstract]
[Full Text]
-
Shaw, R. K., Smollett, K., Cleary, J., Garmendia, J., Straatman-Iwanowska, A., Frankel, G., Knutton, S.
(2005). Enteropathogenic Escherichia coli Type III Effectors EspG and EspG2 Disrupt the Microtubule Network of Intestinal Epithelial Cells. Infect. Immun.
73: 4385-4390
[Abstract]
[Full Text]
-
Tan, Y. P., Zheng, J., Tung, S. L., Rosenshine, I., Leung, K. Y.
(2005). Role of type III secretion in Edwardsiella tarda virulence. Microbiology
151: 2301-2313
[Abstract]
[Full Text]
-
Garmendia, J., Frankel, G., Crepin, V. F.
(2005). Enteropathogenic and Enterohemorrhagic Escherichia coli Infections: Translocation, Translocation, Translocation. Infect. Immun.
73: 2573-2585
[Full Text]
-
Hardwidge, P. R., Deng, W., Vallance, B. A., Rodriguez-Escudero, I., Cid, V. J., Molina, M., Finlay, B. B.
(2005). Modulation of Host Cytoskeleton Function by the Enteropathogenic Escherichia coli and Citrobacter rodentium Effector Protein EspG. Infect. Immun.
73: 2586-2594
[Abstract]
[Full Text]
-
Crepin, V. F., Shaw, R., Abe, C. M., Knutton, S., Frankel, G.
(2005). Polarity of Enteropathogenic Escherichia coli EspA Filament Assembly and Protein Secretion. J. Bacteriol.
187: 2881-2889
[Abstract]
[Full Text]
-
Ritchie, J. M., Waldor, M. K.
(2005). The Locus of Enterocyte Effacement-Encoded Effector Proteins All Promote Enterohemorrhagic Escherichia coli Pathogenicity in Infant Rabbits. Infect. Immun.
73: 1466-1474
[Abstract]
[Full Text]
-
Dahan, S., Wiles, S., La Ragione, R. M., Best, A., Woodward, M. J., Stevens, M. P., Shaw, R. K., Chong, Y., Knutton, S., Phillips, A., Frankel, G.
(2005). EspJ Is a Prophage-Carried Type III Effector Protein of Attaching and Effacing Pathogens That Modulates Infection Dynamics. Infect. Immun.
73: 679-686
[Abstract]
[Full Text]
-
Leverton, L. Q., Kaper, J. B.
(2005). Temporal Expression of Enteropathogenic Escherichia coli Virulence Genes in an In Vitro Model of Infection. Infect. Immun.
73: 1034-1043
[Abstract]
[Full Text]
-
Savkovic, S. D., Villanueva, J., Turner, J. R., Matkowskyj, K. A., Hecht, G.
(2005). Mouse Model of Enteropathogenic Escherichia coli Infection. Infect. Immun.
73: 1161-1170
[Abstract]
[Full Text]
-
Shaw, R. K, Cleary, J., Murphy, M. S., Frankel, G., Knutton, S.
(2005). Interaction of Enteropathogenic Escherichia coli with Human Intestinal Mucosa: Role of Effector Proteins in Brush Border Remodeling and Formation of Attaching and Effacing Lesions. Infect. Immun.
73: 1243-1251
[Abstract]
[Full Text]
-
Nagai, T., Abe, A., Sasakawa, C.
(2005). Targeting of Enteropathogenic Escherichia coli EspF to Host Mitochondria Is Essential for Bacterial Pathogenesis: CRITICAL ROLE OF THE 16TH LEUCINE RESIDUE IN EspF. J. Biol. Chem.
280: 2998-3011
[Abstract]
[Full Text]
-
Berdichevsky, T., Friedberg, D., Nadler, C., Rokney, A., Oppenheim, A., Rosenshine, I.
(2005). Ler Is a Negative Autoregulator of the LEE1 Operon in Enteropathogenic Escherichia coli. J. Bacteriol.
187: 349-357
[Abstract]
[Full Text]
-
Mundy, R., Jenkins, C., Yu, J., Smith, H., Frankel, G.
(2004). Distribution of espI among clinical enterohaemorrhagic and enteropathogenic Escherichia coli isolates. J Med Microbiol
53: 1145-1149
[Abstract]
[Full Text]
-
Dahan, S., Knutton, S., Shaw, R. K., Crepin, V. F., Dougan, G., Frankel, G.
(2004). Transcriptome of Enterohemorrhagic Escherichia coli O157 Adhering to Eukaryotic Plasma Membranes. Infect. Immun.
72: 5452-5459
[Abstract]
[Full Text]
-
Charpentier, X., Oswald, E.
(2004). Identification of the Secretion and Translocation Domain of the Enteropathogenic and Enterohemorrhagic Escherichia coli Effector Cif, Using TEM-1 {beta}-Lactamase as a New Fluorescence-Based Reporter. J. Bacteriol.
186: 5486-5495
[Abstract]
[Full Text]
-
Knappstein, S., Ide, T., Schmidt, M. A., Heusipp, G.
(2004). {alpha}1-Antitrypsin Binds to and Interferes with Functionality of EspB from Atypical and Typical Enteropathogenic Escherichia coli Strains. Infect. Immun.
72: 4344-4350
[Abstract]
[Full Text]
-
Menge, C., Stamm, I., van Diemen, P. M., Sopp, P., Baljer, G., Wallis, T. S., Stevens, M. P.
(2004). Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection. J Med Microbiol
53: 573-579
[Abstract]
[Full Text]
-
Mundy, R., Petrovska, L., Smollett, K., Simpson, N., Wilson, R. K., Yu, J., Tu, X., Rosenshine, I., Clare, S., Dougan, G., Frankel, G.
(2004). Identification of a Novel Citrobacter rodentium Type III Secreted Protein, EspI, and Roles of This and Other Secreted Proteins in Infection. Infect. Immun.
72: 2288-2302
[Abstract]
[Full Text]
-
Campellone, K. G., Rankin, S., Pawson, T., Kirschner, M. W., Tipper, D. J., Leong, J. M.
(2004). Clustering of Nck by a 12-residue Tir phosphopeptide is sufficient to trigger localized actin assembly. JCB
164: 407-416
[Abstract]
[Full Text]
-
Schmidt, H., Hensel, M.
(2004). Pathogenicity Islands in Bacterial Pathogenesis. Clin. Microbiol. Rev.
17: 14-56
[Abstract]
[Full Text]
-
Logsdon, L. K., Mecsas, J.
(2003). Requirement of the Yersinia pseudotuberculosis Effectors YopH and YopE in Colonization and Persistence in Intestinal and Lymph Tissues. Infect. Immun.
71: 4595-4607
[Abstract]
[Full Text]
-
Clarke, S. C., Haigh, R. D., Freestone, P. P. E., Williams, P. H.
(2003). Virulence of Enteropathogenic Escherichia coli, a Global Pathogen. Clin. Microbiol. Rev.
16: 365-378
[Abstract]
[Full Text]
-
Zhou, X., Giron, J. A., Torres, A. G., Crawford, J. A., Negrete, E., Vogel, S. N., Kaper, J. B.
(2003). Flagellin of Enteropathogenic Escherichia coli Stimulates Interleukin-8 Production in T84 Cells. Infect. Immun.
71: 2120-2129
[Abstract]
[Full Text]
-
Neves, B. C., Mundy, R., Petrovska, L., Dougan, G., Knutton, S., Frankel, G.
(2003). CesD2 of Enteropathogenic Escherichia coli Is a Second Chaperone for the Type III Secretion Translocator Protein EspD. Infect. Immun.
71: 2130-2141
[Abstract]
[Full Text]
-
Neves, B. C., Shaw, R. K., Frankel, G., Knutton, S.
(2003). Polymorphisms within EspA Filaments of Enteropathogenic and Enterohemorrhagic Escherichia coli. Infect. Immun.
71: 2262-2265
[Abstract]
[Full Text]
-
Jores, J., Zehmke, K., Eichberg, J., Rumer, L., Wieler, L. H.
(2003). Description of a Novel Intimin Variant (Type {zeta}) in the Bovine O84:NM Verotoxin-Producing Escherichia coli Strain 537/89 and the Diagnostic Value of Intimin Typing. Exp. Biol. Med.
228: 370-376
[Abstract]
[Full Text]
-
Kenny, B.
(2002). Enteropathogenic Escherichia coli (EPEC) - a crafty subversive little bug. Microbiology
148: 1967-1978
[Full Text]
-
Elliott, S. J., O'Connell, C. B., Koutsouris, A., Brinkley, C., Donnenberg, M. S., Hecht, G., Kaper, J. B.
(2002). A Gene from the Locus of Enterocyte Effacement That Is Required for Enteropathogenic Escherichia coli To Increase Tight-Junction Permeability Encodes a Chaperone for EspF. Infect. Immun.
70: 2271-2277
[Abstract]
[Full Text]
-
Deng, W., Li, Y., Vallance, B. A., Finlay, B. B.
(2001). Locus of Enterocyte Effacement from Citrobacter rodentium: Sequence Analysis and Evidence for Horizontal Transfer among Attaching and Effacing Pathogens. Infect. Immun.
69: 6323-6335
[Abstract]
[Full Text]