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Infection and Immunity, June 2001, p. 4129-4133, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.4129-4133.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mapping the Ligand-Binding Region of Borrelia burgdorferi Fibronectin-Binding Protein BBK32

William S. Probert,1,dagger Jung Hwa Kim,2 Magnus Höök,2 and Barbara J. B. Johnson1,*

Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522,1 and Center for Extracellular Matrix Biology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M University, Houston, Texas 770302

Received 20 July 2000/Returned for modification 26 October 2000/Accepted 2 March 2001

The cellular attachment and entry of pathogenic microorganisms can be facilitated by the expression of microbial adhesins that bind fibronectin. We have previously described a Borrelia burgdorferi gene, bbk32, that encodes a 47-kDa fibronectin-binding protein. In this study, the ligand-binding region of BBK32 from B. burgdorferi isolate B31 was localized to 32 amino acids. The bbk32 gene was cloned and sequenced from three additional B. burgdorferi isolates representing different genospecies of B. burgdorferi sensu lato. All four bbk32 genes encoded proteins having fibronectin-binding activity when expressed in Escherichia coli, and the deduced proteins shared 81 to 91% amino acid sequence identity within the ligand-binding domain. In addition, the ligand-binding region of BBK32 was found to share sequence homology with a fibronectin-binding peptide defined for protein F1 of Streptococcus pyogenes. The structural and functional similarity between the ligand-binding region of BBK32 and the UR region of protein F1 suggests a common mechanism of cellular adhesion and entry for B. burgdorferi and S. pyogenes.

* Corresponding author. Mailing address: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Ft. Collins, CO 80522. Phone: (970) 221-6463. Fax: (970) 221-6476. E-mail: bjj1{at}cdc.gov.

dagger Present address: California Department of Health Services, Microbial Diseases Laboratory, Berkeley, CA 94704.

Infection and Immunity, June 2001, p. 4129-4133, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.4129-4133.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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