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Infection and Immunity, June 2001, p. 4154-4158, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.4154-4158.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of a Novel T-Cell Epitope in Soluble Egg Antigen of Schistosoma japonicum

Jie Liu,1 Kachio Tasaka,1,* Junqi Yang,1,dagger Takehito Itoh,2 Muneo Yamada,2 Hideshi Yoshikawa,1 and Yasuo Nakajima1

Department of Parasitology and Immunology, Yamanashi Medical University, Yamanashi 409-3898,1 and Food R&D Laboratory, Morinaga Milk Industry, Zama, Kanagawa 228-8583,2 Japan

Received 2 January 2001/Returned for modification 9 February 2001/Accepted 7 March 2001

Identification of T-cell epitopes harbored in soluble egg antigen (SEA) of Schistosoma japonicum and study of the immunological properties are essential for understanding the immunopathology and the control of schistosomiasis. As a follow-up to our previous work, the 66- to 80-kDa fragment from SEA was partially digested with protease, fractionated by reverse-phase high-pressure liquid chromatography, and found to be carrying a peptide which stimulated proliferation and gamma interferon (IFN-gamma ) production of Th1 clones specific to SEA. Sequence analysis showed that the peptide was composed of 12 amino acids lined up as DLAVELAYLGNL. A synthetic homologue induced proliferation and IFN-gamma and interleukin-2 (IL-2) production, but not IL-4 or IL-6 production, by the Th1 clones as well as by the spleen cells from SEA-immunized mice, thus indicating that the peptide carries a Th1 epitope of SEA.

* Corresponding author. Mailing address: Department of Parasitology and Immunology, Yamanashi Medical University, Yamanashi 409-3898, Japan. Phone: (81)-55-273-9541. Fax: (81)-55-273-9542. E-mail: ktasaka{at}res.yamanashi-med.ac.jp.

dagger Present address: Division of Immunology, University of Cincinnati, Cincinnati, OH 45267-0563.

Infection and Immunity, June 2001, p. 4154-4158, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.4154-4158.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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