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Infection and Immunity, June 2001, p. 4168-4173, Vol. 69, No. 6
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.6.4168-4173.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
-Glutamyltransferase Is a Helicobacter
pylori Virulence Factor but Is Not Essential for
Colonization
K. J.
McGovern,1
T. G.
Blanchard,2,*
J. A.
Gutierrez,1
S. J.
Czinn,2
S.
Krakowka,3 and
P.
Youngman1
Millennium Pharmaceuticals, Inc., Cambridge,
Massachusetts,1 and Department of
Pediatrics, Case Western Reserve University School of Medicine,
Cleveland,2 and Department of
Veterinary Biosciences, Ohio State University,
Columbus,3 Ohio
Received 28 November 2000/Returned for modification 12 January
2001/Accepted 8 March 2001
The contribution of glutamyl transpeptidase (GGT)
(
-glutamyltransferase [EC 2. 3. 2. 2]) to Helicobacter
pylori virulence was investigated in piglets and mice using
GGT-deficient isogenic strains. All animals became colonized. However,
the bacterial load was significantly lower for mutant bacteria than for
parent strains. These results suggest that GGT activity provides an
advantage to H. pylori in colonization.
*
Corresponding author. Mailing address: Division of
Gastroenterology, Rainbow Babies & Children's Hospital, Case Western
Reserve University School of Medicine, Cleveland, OH 44106. Phone:
(216) 844-7363. Fax: (216) 844-7642. E-mail:
tgb4{at}po.cwru.edu.
Infection and Immunity, June 2001, p. 4168-4173, Vol. 69, No. 6
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.6.4168-4173.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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