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Infection and Immunity, June 2001, p. 4185-4191, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.4185-4191.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Antigens of Mycobacterium tuberculosis Expressed during Preclinical Tuberculosis: Serological Immunodominance of Proteins with Repetitive Amino Acid Sequences

K. K. Singh,1 X. Zhang,1 A. S. Patibandla,2 P. Chien Jr.,1 and S. Laal1,3,*

Department of Pathology, New York University Medical Center, New York, New York 100161; Bayer Diagnostics, Tarrytown, New York 105912; and Research Center for AIDS and HIV Infection, Veterans Affairs Medical Center, New York, New York 100103

Received 27 November 2000/Returned for modification 29 January 2001/Accepted 7 March 2001

Four antigens of Mycobacterium tuberculosis that are expressed in vivo after aerosol infection but prior to the development of clinical tuberculosis (TB) in rabbits were identified by immunoscreening of an expression library of M. tuberculosis genomic DNA with sera obtained 5 weeks postinfection. Three of the proteins identified, PirG (Rv3810), polymorphic GC-repetitive sequence (PE-PGRS; Rv3367), and proline-threonine repetitive protein (PTRP) (Rv0538), have multiple tandem repeats of unique amino acid sequences and have characteristics of surface or secreted proteins. The fourth protein, MtrA (Rv3246c), is a response regulator of a putative two-component signal transduction system, mtrA-mtrB, of M. tuberculosis. All four antigens were recognized by pooled sera from TB patients and not from healthy controls, confirming their in vivo expression during active infection in humans. Three of the antigens (PE-PGRS, PTRP, and MtrA) were also recognized by retrospective preclinical TB sera obtained, prior to the clinical manifestation of TB, from human immunodeficiency virus-TB patients, suggesting that they are potential candidates for devising diagnostic tests for active, preclinical TB.

* Corresponding author. Mailing address: Veterans Affairs Medical Center, Research Center for AIDS and HIV Infection, Room 18123 North, 423 East 23rd St., New York, NY 10010. Phone: (212) 263-6769. Fax: (212) 951-6321. E-mail: suman.laal{at}med.nyu.edu.

Infection and Immunity, June 2001, p. 4185-4191, Vol. 69, No. 6
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.6.4185-4191.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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