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Infection and Immunity, July 2001, p. 4268-4275, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4268-4275.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Interleukin-6 Enhances Production of Anti-OspC Immunoglobulin G2b Borreliacidal Antibody

Monica C. Remington,1,2 Erik L. Munson,1,2 Steven M. Callister,3,4 Melanie L. Molitor,1,2 John A. Christopherson,1,2 David J. DeCoster,1,2 Steven D. Lovrich,3 and Ronald F. Schell1,2,5,*

Wisconsin State Laboratory of Hygiene1 and Departments of Medical Microbiology and Immunology2 and Bacteriology,5 University of Wisconsin, Madison, Wisconsin 53706, and Microbiology Research Laboratory3 and Section of Infectious Diseases,4 Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601

Received 14 November 2000/Returned for modification 23 February 2001/Accepted 29 March 2001

Protection against infection with Borrelia burgdorferi is dependent primarily on induction of complement-dependent antibody that can kill the spirochete. Measuring the production of sustained high levels of borreliacidal antibody is thus paramount for determining potential vaccine efficacy. We investigated the borreliacidal antibody response in sera and the amount of antibody produced by cultured lymph node cells of C3H/HeJ mice vaccinated with outer surface protein C (OspC). We showed that recombinant OspC was a weak stimulant of borreliacidal antibody production compared to whole cells of OspC-expressing B. burgdorferi. Mice vaccinated with B. burgdorferi in adjuvant produced a high level (titer, 5,120) of anti-OspC borreliacidal antibody, which waned rapidly. Similarly, borreliacidal antibody production by cultured lymph node cells from vaccinated mice peaked soon after vaccination and then decreased. Treatment of lymph node cells with interleukin-6 (IL-6) augmented borreliacidal antibody production, particularly immunoglobulin G2b, whereas treatment with anti-IL-6 inhibited the borreliacidal response. These findings demonstrate a previously unrecognized role for IL-6 in borreliacidal antibody production that may have important implications for vaccine development.

* Corresponding author. Mailing address: University of Wisconsin, Wisconsin State Laboratory of Hygiene, 465 Henry Mall, Madison, WI 53706. Phone: (608) 262-3634. Fax: (608) 265-3451. E-mail: RFSchell{at}Facstaff.wisc.edu.

Infection and Immunity, July 2001, p. 4268-4275, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4268-4275.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.