Impact of Genotypic Variation of Borrelia burgdorferi Sensu Stricto on Kinetics of Dissemination and Severity of Disease in C3H/HeJ Mice

doi:10.1128/IAI.69.7.4303-4312.2001

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Infection and Immunity, July 2001, p. 4303-4312, Vol. 69, No. 7
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.7.4303-4312.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Impact of Genotypic Variation of Borrelia burgdorferi Sensu Stricto on Kinetics of Dissemination and Severity of Disease in C3H/HeJ Mice

Guiqing Wang,¹ Caroline Ojaimi,¹ Radha Iyer,¹ Victoria Saksenberg,² Steve A. McClain,² Gary P. Wormser,³ and Ira Schwartz¹^,³^,^*

Department of Biochemistry and Molecular Biology¹ and Division of Infectious Diseases,³ Department of Medicine, New York Medical College, Valhalla, New York 10595, and Department of Pathology, Montefiore Medical Center, Bronx, New York 10467²

Received 13 December 2000/Returned for modification 16 February 2001/Accepted 29 March 2001

Various genotypes of Borrelia burgdorferi sensu stricto have been previously identified among a large collection of isolatescultured from patients with Lyme disease in the United States.Furthermore, association of specific genotypes with hematogenousdissemination early in the disease course has been observed. Thepresent study assessed kinetics of spirochete dissemination anddisease severity in C3H/HeJ mice infected with two different genotypesof B. burgdorferi. Spirochete load in plasma and ear and othertissue samples of infected mice was measured by quantitative PCR,and these data were compared to those obtained by culture andhistopathologic analysis. In mice infected with isolate BL206(a type 1 strain), the peak number of spirochetes was observedin plasma between day 4 and 7, in heart and ear tissue on day14, and in joints on day 28 postinoculation. There was a correlationbetween the peak number of spirochetes in plasma on day 4 or 7and that in ear biopsy and joint specimens on day 14. By contrast,spirochete burdens in plasma of mice infected with isolate B356(a type 3 strain) were 16- and 5-fold lower than those of BL206-infectedmice on days 7 and 14 of infection, respectively. Similarly, approximately6- and 13-fold fewer spirochetes were detected in the heart tissuesof B356-infected mice compared to BL206-infected mice. Histopathologically,severe arthritis and aortitis were noted only in mice infectedwith isolate BL206. Spirochete dissemination and disease severityvary significantly in mice infected with distinct genotypes ofB. burgdorferi, suggesting that genotypic differences in the infectingspirochetes play a key role in the pathogenesis and developmentof clinicaldisease.

^* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla,NY 10595. Phone: (914) 594-4658. Fax: (914) 594-3455. E-mail:schwartz{at}nymc.edu.

Infection and Immunity, July 2001, p. 4303-4312, Vol. 69, No. 7
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.7.4303-4312.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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