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Infection and Immunity, July 2001, p. 4600-4609, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4600-4609.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Homogeneity of Antibody Responses in Tuberculosis Patients

K. Samanich,1 J. T. Belisle,2 and S. Laal1,3,*

Department of Pathology, New York University School of Medicine, New York, New York 100161; Department of Microbiology, Colorado State University, Fort Collins, Colorado 805232; and Research Center for AIDS and HIV Infection, Veterans Affairs Medical Center, New York, New York 100103

Received 16 November 2000/Returned for modification 16 January 2001/Accepted 16 March 2001

The goals of the present study were twofold: (i) to compare the repertoires of antigens in culture filtrates of in vitro-grown Mycobacterium tuberculosis that are recognized by antibodies from noncavitary and cavitary tuberculosis (TB) patients and (ii) to determine the extent of variation that exists between the antigen profiles recognized by individual TB patients. Lipoarabinomannan-free culture filtrate proteins of M. tuberculosis were fractionated by one-dimensional (1-D) and 2-D polyacrylamide gel electrophoresis, and the Western blots were probed with sera from non-human immunodeficiency virus (non-HIV)-infected cavitary and noncavitary TB patients and from HIV-infected, noncavitary TB patients. In contrast to earlier studies based on recombinant antigens of M. tuberculosis which suggested that antibody responses in TB patients were heterogeneous (K. Lyashchenko et al., 1998, Infect. Immun. 66:3936-3940, 1998), our studies with native culture filtrate proteins show that the antibody responses in TB patients show significant homogeneity in being directed against a well-defined subset of antigens. Thus, there is a well-defined subset of culture filtrate antigens that elicits antibodies during noncavitary and cavitary disease. In addition, another set of antigens is recognized primarily by cavitary TB patients. The mapping with individual patient sera presented here suggests that serodiagnostic tests based on the subset of antigens recognized during both noncavitary and cavitary TB will enhance the sensitivity of antibody detection in TB patients, especially in difficult-to-diagnose, smear-negative, noncavitary TB patients.

* Corresponding author. Mailing address: Research Center for AIDS and HIV Infection, Veterans Affairs Medical Center, Room 18123 North, 423 East 23rd St., New York, NY 10010. Phone: (212) 263-6769. Fax: (212) 951-6321. E-mail: Suman.Laal{at}Med.Nyu.Edu.

Infection and Immunity, July 2001, p. 4600-4609, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4600-4609.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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