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Infection and Immunity, July 2001, p. 4627-4638, Vol. 69, No. 7
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.7.4627-4638.2001
Complete DNA Sequence of Yersinia
enterocolitica Serotype 0:8 Low-Calcium-Response Plasmid
Reveals a New Virulence Plasmid-Associated Replicon
Norma J.
Snellings,*
Michael
Popek, and
Luther E.
Lindler
Department of Bacterial Diseases, Division of
Communicable Diseases and Immunology, Walter Reed Army Institute of
Research, Silver Spring, Maryland 20910-7500
Received 28 December 2000/Returned for modification 14 March
2001/Accepted 18 April 2001
The complete nucleotide sequence and organization of the
Yersinia enterocolitica serotype 0:8
low-calcium-response (LCR) plasmid, pYVe8081, were determined. The
67,720-bp plasmid encoded all the genes known to be part of the LCR
stimulon except for ylpA. Eight of 13 intact open
reading frames of unknown function identified in pYVe8081 had
homologues in Yersinia pestis plasmid pCD1 or in
Y. enterocolitica serotype 0:9 plasmid pYVe227.
A region of approximately 17 kbp showed no DNA identity to pCD1 or
pYVe227 and contained six potential new genes, a possible new replicon, and two intact insertion sequence (IS) elements. One intact IS element,
ISYen1, was a new IS belonging to the
IS256 family. Several vestigial IS elements appeared
different from the IS distribution seen in the other LCR plasmids. The
RepA proteins encoded by Y. enterocolitica
serotype 0:8 pYVeWA and pYVe8081 were identical. The putative
pYVe8081 replicon showed significant homology to the IncL/M replicon of
pMU407.1 but was only distantly related to the replicons of pCD1 and
pYVe227. In contrast, the putative partitioning genes of pYVe8081
showed 97% DNA identity to the spy/sopABC loci
of pCD1 and pYVe227. Sequence analysis suggests that
Yersinia LCR plasmids are from a common ancestor but
that Y. enterocolitica serotype 0:8 plasmid replicons
may have evolved independently via cointegrate formation following a
transposition event. The change in replicon structure is predicted to
change the incompatibility properties of Y.
enterocolitica serotype 0:8 plasmids from those of Y.
enterocolitica serotype 0:9 and Y. pestis LCR plasmids.
*
Corresponding author. Mailing address: Department of
Bacterial Diseases, WRAIR, Room 3A08, 503 Robert Grant Ave., Silver
Spring, MD 20910-7500. Phone: (301) 319-9825. Fax: (301) 319-9123. E-mail: norma.snellings{at}na.amedd.army.mil.
Infection and Immunity, July 2001, p. 4627-4638, Vol. 69, No. 7
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.7.4627-4638.2001
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