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Infection and Immunity, July 2001, p. 4647-4653, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4647-4653.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression and Functional Properties of the Streptococcus intermedius Surface Protein Antigen I/II

F.C. Petersen,1,* S. Pasco,2 J. Ogier,2 J. P. Klein,3 S. Assev,1 and A. A. Scheie1

Department of Oral Biology, Dental Faculty, University of Oslo, Blindern, N0372 Oslo, Norway,1 and INSERM U424, F-67085 Strasbourg Cédex,2 and INSERM U392, Faculté de Pharmacie, F-67401 Illkirch Cédex,3 France

Received 7 November 2000/Returned for modification 5 February 2001/Accepted 10 April 2001

Streptococcus intermedius is associated with deep-seated purulent infections. In this study, we investigated expression and functional activities of antigen I/II in S. intermedius. The S. intermedius antigen I/II appeared to be cell surface associated, with a molecular mass of approximately 160 kDa. Northern blotting indicated that the S. intermedius NCTC 11324 antigen I/II gene was transcribed as a monocistronic message. Maximum expression was seen during the early exponential phase. Insertional inactivation of the antigen I/II gene resulted in reduced hydrophobicity during early exponential phase, whereas no effect was detected during mid- and late exponential phases. Binding to human fibronectin and laminin was reduced in the isogenic mutant, whereas binding to human collagen types I and IV and to rat collagen type I was not significant for either the wild type or the mutant. Compared to the wild type, the capacity of the isogenic mutant to induce interleukin 8 (IL-8) release by THP-1 monocytic cells was significantly reduced. The results indicate that the S. intermedius antigen I/II is involved in adhesion to human receptors and in IL-8 induction.

* Corresponding author. Mailing address: Department of Oral Biology, Dental Faculty, University of Oslo, P.O. Box 1052, Sognsvannsveien 11, Blindern, N0372 Oslo, Norway. Phone: 47 22840352. Fax: 47 22840302. E-mail: cpaiva{at}odont.uio.no.

Infection and Immunity, July 2001, p. 4647-4653, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4647-4653.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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