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Infection and Immunity, July 2001, p. 4691-4694, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4691-4694.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Francisella tularensis Induces Cytopathogenicity and Apoptosis in Murine Macrophages via a Mechanism That Requires Intracellular Bacterial Multiplication

Xin-He Lai,dagger Igor Golovliov, and Anders Sjöstedt*

Department of Clinical Microbiology, Clinical Bacteriology, Umeå University, SE-901 85 Umeå, Sweden

Received 8 January 2001/Returned for modification 12 February 2001/Accepted 2 April 2001

The murine macrophage-like cell line J774.A1 ingests and allows intracellular growth of Francisella tularensis. We demonstrate that, after 24 h of infection, a pronounced cytopathogenicity resulted and the J774 cells were undergoing apoptosis. Despite this host cell apoptosis, no decrease in bacterial numbers was observed. When internalization of bacteria was prevented or intracellularly located F. tularensis bacteria were eradicated within 12 h, the progression of host cell cytopathogenicity and apoptosis was prevented.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Clinical Bacteriology, Umeå University, SE-901 85 Umeå, Sweden. Phone: 46 90 7851120. Fax: 46 90 7852225. E-mail: anders.sjostedt{at}climi.umu.se.

dagger Permanent address: Priority Laboratory of Molecular Medical Bacteriology of Ministry of Public Health, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing, China.

Infection and Immunity, July 2001, p. 4691-4694, Vol. 69, No. 7
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.7.4691-4694.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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