This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rokbi, B.
Right arrow Articles by Quentin-Millet, M.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rokbi, B.
Right arrow Articles by Quentin-Millet, M.-J.

 Previous Article  |  Next Article 

Infection and Immunity, August 2001, p. 4759-4766, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.4759-4766.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Assessment of Helicobacter pylori Gene Expression within Mouse and Human Gastric Mucosae by Real-Time Reverse Transcriptase PCR

Bachra Rokbi,1,* Delphine Seguin,1 Bruno Guy,1 Véronique Mazarin,1 Emmanuel Vidor,1 François Mion,2 Michel Cadoz,1 and Marie-José Quentin-Millet1

Aventis Pasteur1 and Fédération des Spécialités Digestives, Hôpital Edouard Herriot,2 Lyon, France

Received 28 March 2001/Accepted 2 May 2001

Despite increasing knowledge on the biology of Helicobacter pylori, little is known about the expression pattern of its genome during infection. While mouse models of infection have been widely used for the screening of protective antigens, the reliability of the mouse model for gene expression analysis has not been assessed. In an attempt to address this question, we have developed a quantitative reverse transcriptase PCR (RT-PCR) that allowed the detection of minute amounts of mRNA within the gastric mucosa. The expression of four genes, 16S rRNA, ureA (encoding urease A subunit), katA (catalase), and alpA (an adhesin), was monitored during the course of a 6-month infection of mice and in biopsy samples from of 15 infected humans. We found that the selected genes were all expressed within both mouse and human infected mucosae. Moreover, the relative abundance of transcripts was the same (16S rRNA > ureA > katA > alpA), in the two models. Finally, results obtained with the mouse model suggest a negative effect of bacterial burden on the number of transcripts of each gene expressed per CFU (P < 0.05 for 16S rRNA, alpA, and katA). Overall, this study demonstrates that real-time RT-PCR is a powerful tool for the detection and quantification of H. pylori gene expression within the gastric mucosa and strongly indicates that mice experimentally infected with H. pylori provide a valuable model for the analysis of bacterial gene expression during infection.

* Corresponding author. Mailing address: Aventis Pasteur, Campus Mérieux, 1541 avenue Marcel Mérieux, 69280 Marcy L'Etoile. Phone: (33)04.37.37.36.05. Fax: (33)04.37.37.31.89. E-mail: Bachra.Rokbi{at}aventis.com.

Infection and Immunity, August 2001, p. 4759-4766, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.4759-4766.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lee, S.-B., Yang, J.-W., Kim, C.-S. (2008). The association between conjunctival MALT lymphoma and Helicobacter pylori. Br J Ophthalmol 92: 534-536 [Abstract] [Full Text]  
  • Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
  • Bron, P. A., Monk, I. R., Corr, S. C., Hill, C., Gahan, C. G. M. (2006). Novel Luciferase Reporter System for In Vitro and Organ-Specific Monitoring of Differential Gene Expression in Listeria monocytogenes. Appl. Environ. Microbiol. 72: 2876-2884 [Abstract] [Full Text]  
  • Oozeer, R., Furet, J. P., Goupil-Feuillerat, N., Anba, J., Mengaud, J., Corthier, G. (2005). Differential Activities of Four Lactobacillus casei Promoters during Bacterial Transit through the Gastrointestinal Tracts of Human-Microbiota-Associated Mice. Appl. Environ. Microbiol. 71: 1356-1363 [Abstract] [Full Text]  
  • Fey, A., Eichler, S., Flavier, S., Christen, R., Hofle, M. G., Guzman, C. A. (2004). Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism. Appl. Environ. Microbiol. 70: 3618-3623 [Abstract] [Full Text]  
  • de Jonge, R., Durrani, Z., Rijpkema, S. G., Kuipers, E. J., van Vliet, A. H.M., Kusters, J. G. (2004). Role of the Helicobacter pylori outer-membrane proteins AlpA and AlpB in colonization of the guinea pig stomach. J Med Microbiol 53: 375-379 [Abstract] [Full Text]  
  • Zoetendal, E. G., Collier, C. T., Koike, S., Mackie, R. I., Gaskins, H. R. (2004). Molecular Ecological Analysis of the Gastrointestinal Microbiota: A Review. J. Nutr. 134: 465-472 [Abstract] [Full Text]  
  • Backhed, F., Torstensson, E., Seguin, D., Richter-Dahlfors, A., Rokbi, B. (2003). Helicobacter pylori Infection Induces Interleukin-8 Receptor Expression in the Human Gastric Epithelium. Infect. Immun. 71: 3357-3360 [Abstract] [Full Text]  
  • Virtaneva, K., Graham, M. R., Porcella, S. F., Hoe, N. P., Su, H., Graviss, E. A., Gardner, T. J., Allison, J. E., Lemon, W. J., Bailey, J. R., Parnell, M. J., Musser, J. M. (2003). Group A Streptococcus Gene Expression in Humans and Cynomolgus Macaques with Acute Pharyngitis. Infect. Immun. 71: 2199-2207 [Abstract] [Full Text]  
  • Angelichio, M. J., Camilli, A. (2002). In Vivo Expression Technology. Infect. Immun. 70: 6518-6523 [Full Text]  
  • Bumann, D., Holland, P., Siejak, F., Koesling, J., Sabarth, N., Lamer, S., Zimny-Arndt, U., Jungblut, P. R., Meyer, T. F. (2002). A Comparison of Murine and Human Immunoproteomes of Helicobacter pylori Validates the Preclinical Murine Infection Model for Antigen Screening. Infect. Immun. 70: 6494-6498 [Abstract] [Full Text]  
  • Sabarth, N., Hurwitz, R., Meyer, T. F., Bumann, D. (2002). Multiparameter Selection of Helicobacter pylori Antigens Identifies Two Novel Antigens with High Protective Efficacy. Infect. Immun. 70: 6499-6503 [Abstract] [Full Text]  
  • Bjorkholm, B. M., Guruge, J. L., Oh, J. D., Syder, A. J., Salama, N., Guillemin, K., Falkow, S., Nilsson, C., Falk, P. G., Engstrand, L., Gordon, J. I. (2002). Colonization of Germ-free Transgenic Mice with Genotyped Helicobacter pylori Strains from a Case-Control Study of Gastric Cancer Reveals a Correlation between Host Responses and HsdS Components of Type I Restriction-Modification Systems. J. Biol. Chem. 277: 34191-34197 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»