This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heneghan, M. A.
Right arrow Articles by Moran, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heneghan, M. A.
Right arrow Articles by Moran, A. P.

 Previous Article  |  Next Article 

Infection and Immunity, August 2001, p. 4774-4781, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.4774-4781.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Relationship of Anti-Lewis x and Anti-Lewis y Antibodies in Serum Samples from Gastric Cancer and Chronic Gastritis Patients to Helicobacter pylori-Mediated Autoimmunity

Michael A. Heneghan,1,2,dagger Ciaran F. McCarthy,1 Daiva Janulaityte,3 and Anthony P. Moran2,*

Department of Medicine, Clinical Science Institute, University College Hospital Galway,1 and Laboratory of Molecular Biochemistry, Department of Microbiology,2 National University of Ireland, Galway, Ireland, and Department of Microbiology, Kaunas Medical Academy, Kaunas, Lithuania3

Received 27 June 2000/Returned for modification 7 September 2000/Accepted 7 May 2001

Lewis (Le) antigens have been implicated in the pathogenesis of atrophic gastritis and gastric cancer in the setting of Helicobacter pylori infection, and H. pylori-induced anti-Le antibodies have been described that cross-react with the gastric mucosa of both mice and humans. The aim of this study was to examine the presence of anti-Le antibodies in patients with H. pylori infection and gastric cancer and to examine the relationships between anti-Le antibody production, bacterial Le expression, gastric histopathology, and host Le erythrocyte phenotype. Anti-Le antibody production and H. pylori Le expression were determined by enzyme-linked immunosorbent assay, erythrocyte Le phenotype was examined by agglutination assays, and histology was scored blindly. Significant levels of anti-Lex antibody (P < 0.0001, T = 76.4, DF = 5) and anti-Ley antibody (P < 0.0001, T = 73.05, DF = 5) were found in the sera of patients with gastric cancer and other H. pylori-associated pathology compared with H. pylori-negative controls. Following incubation of patient sera with synthetic Le glycoconjugates, anti-Lex and -Ley autoantibody binding was abolished. The degree of the anti-Lex and -Ley antibody response was unrelated to the host Le phenotype but was significantly associated with the bacterial expression of Lex (r = 0.863, r2 = 0.745, P < 0.0001) and Ley (r = 0.796, r2 = 0.634, P < 0.0001), respectively. Collectively, these data suggest that anti-Le antibodies are present in most patients with H. pylori infection, including those with gastric cancer, that variability exists in the strength of the anti-Le response, and that this response is independent of the host Le phenotype but related to the bacterial Le phenotype.

* Corresponding author. Mailing address: Laboratory of Molecular Biochemistry, Department of Microbiology, National University of Ireland, Galway, University Road, Galway, Ireland. Phone: 353-91-524411, x3163. Fax: 353-91-525700. E-mail: anthony.moran{at}nuigalway.ie.

dagger Present address: Division of Gastroenterology, Duke University Medical Center, Durham, NC 27710.

Infection and Immunity, August 2001, p. 4774-4781, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.4774-4781.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sheu, S.-M., Sheu, B.-S., Yang, H.-B., Lei, H.-Y., Wu, J.-J. (2007). Anti-Lewis X Antibody Promotes Helicobacter pylori Adhesion to Gastric Epithelial Cells. Infect. Immun. 75: 2661-2667 [Abstract] [Full Text]  
  • Ma, B., Simala-Grant, J. L., Taylor, D. E. (2006). Fucosylation in prokaryotes and eukaryotes. Glycobiology 16: 158R-184R [Abstract] [Full Text]  
  • Nakagawa, S., Osaki, T., Fujioka, Y., Yamaguchi, H., Kamiya, S. (2005). Long-Term Infection of Mongolian Gerbils with Helicobacter pylori: Microbiological, Histopathological, and Serological Analyses. CVI 12: 347-353 [Abstract] [Full Text]  
  • Eaton, K. A., Logan, S. M., Baker, P. E., Peterson, R. A., Monteiro, M. A., Altman, E. (2004). Helicobacter pylori with a Truncated Lipopolysaccharide O Chain Fails To Induce Gastritis in SCID Mice Injected with Splenocytes from Wild-Type C57BL/6J Mice. Infect. Immun. 72: 3925-3931 [Abstract] [Full Text]  
  • Reiche, N., Jung, A., Brabletz, T., Vater, T., Kirchner, T., Faller, G. (2002). Generation and Characterization of Human Monoclonal scFv Antibodies against Helicobacter pylori Antigens. Infect. Immun. 70: 4158-4164 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»