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Infection and Immunity, August 2001, p. 4816-4822, Vol. 69, No. 8
Unité de Recherche en Biologie
Moléculaire, Laboratoire d'Immunologie et de Microbiologie,
Facultés Universitaires Notre-Dame de la Paix, B-5000
Namur,1 and Centre d'Etude et de
Recherche Vétérinaire et Agrochimique, B-1180
Brussels,2 Belgium
Received 28 February 2001/Returned for modification 11 April
2001/Accepted 7 May 2001
The P39 and the bacterioferrin (BFR) antigens of Brucella
melitensis 16M were previously identified as T dominant antigens able to induce both delayed-type hypersensivity in sensitized guinea
pigs and in vitro gamma interferon (IFN-
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.8.4816-4822.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Protection of BALB/c Mice against Brucella
abortus 544 Challenge by Vaccination with Bacterioferritin or
P39 Recombinant Proteins with CpG Oligodeoxynucleotides as
Adjuvant
) production by peripheral
blood mononuclear cells from infected cattle. Here, we analyzed the
potential for these antigens to function as a subunitary vaccine
against Brucella abortus infection in BALB/c mice, and we
characterized the humoral and cellular immune responses induced. Mice
were injected with each of the recombinant proteins alone or adjuvanted
with either CpG oligodeoxynucleotides (CpG ODN) or non-CpG ODN. Mice
immunized with the recombinant antigens with CpG ODN were the only
group demonstrating both significant IFN-
production and T-cell
proliferation in response to either Brucella extract or to
the respective antigen. The same conclusion holds true for the antibody
response, which was only demonstrated in mice immunized with
recombinant antigens mixed with CpG ODN. The antibody titers (both
immunoglobulin G1 [IgG1] and IgG2a) induced by P39 immunization were
higher than the titers induced by BFR (only IgG2a). Using a B. abortus 544 challenge, the level of protection was analyzed and
compared to the protection conferred by one immunization with the
vaccine strain B19. Immunization with P39 and CpG ODN gave a level of
protection comparable to the one conferred by B19 at 4 weeks
postchallenge, and the mice were still significantly protected at 8 weeks postchallenge, although to a lesser extent than the
B19-vaccinated group. Intriguingly, no protection was detected after
BFR vaccination. All other groups did not demonstrate any protection.
*
Corresponding author. Mailing address. Unité de
Recherche en Biologie Moléculaire (URBM), Laboratoire
d'Immunologie et de Microbiologie, Facultés Universitaires
Notre-Dame de la Paix, Rue de Bruxelles 61, B-5000 Namur, Belgium.
Phone: 32-81-72-44-02. Fax: 32-81-72-42-97. E-mail:
jean-jacqnes.letesson{at}fundp.ac.be.
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