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Infection and Immunity, August 2001, p. 4870-4873, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.4870-4873.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Intranasal Immunization with Killed Unencapsulated Whole Cells Prevents Colonization and Invasive Disease by Capsulated Pneumococci

Richard Malley,1,2,* Marc Lipsitch,3 Anne Stack,2 Richard Saladino,2 Gary Fleisher,2 Steven Pelton,4 Claudette Thompson,3 David Briles,5 and Porter Anderson6

Divisions of Infectious Diseases1 and Emergency Medicine,2 Department of Medicine, Children's Hospital, Harvard School of Public Health, Harvard University,3 and Division of Pediatric Infectious Diseases, Boston Medical Center and Boston University,4 Boston, Massachusetts; Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama5; and Department of Pediatrics, University of Rochester, Rochester, New York6

Received 2 March 2001/Returned for modification 29 March 2001/Accepted 1 May 2001

A whole-cell killed unencapsulated pneumococcal vaccine given by the intranasal route with cholera toxin as an adjuvant was tested in two animal models. This vaccination was highly effective in preventing nasopharyngeal colonization with an encapsulated serotype 6B strain in mice and also conferred protection against illness and death in rats inoculated intrathoracically with a highly encapsulated serotype 3 strain. When the serotype 3 challenge strain was incubated in the sera of immunized rats, it was no longer virulent in an infant-rat sepsis model, indicating that the intranasal immunization elicited protective systemic antibodies. These studies suggest that killed whole-cell unencapsulated pneumococci given intranasally with an adjuvant may provide multitypic protection against capsulated pneumococci.

* Corresponding author. Mailing address: Divisions of Infectious Diseases and Emergency Medicine, Children's Hospital, 300 Longwood Ave., Boston, MA 02115. Phone: (617) 355-7456. Fax: (617) 355-6625. E-mail: richard.malley{at}tch.harvard.edu.

Infection and Immunity, August 2001, p. 4870-4873, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.4870-4873.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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