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Infection and Immunity, August 2001, p. 4898-4905, Vol. 69, No. 8
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.8.4898-4905.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Neutrophil Depletion during Toxoplasma
gondii Infection Leads to Impaired Immunity and Lethal
Systemic Pathology
Susan K.
Bliss,1
L. Cristina
Gavrilescu,1
Ana
Alcaraz,2 and
Eric Y.
Denkers1,*
Departments of Microbiology and
Immunology1 and Population Medicine and
Diagnostic Sciences,2 College of Veterinary
Medicine, Cornell University, Ithaca, New York 14853-6401
Received 14 February 2001/Returned for modification 27 March
2001/Accepted 14 May 2001
The immunomodulatory role of neutrophils during infection with
Toxoplasma gondii was investigated. Monoclonal
antibody-mediated depletion revealed that neutrophils are essential for
survival during the first few days of infection. Moreover, neutrophil
depletion was associated with a weaker type 1 immune response as
measured by decreased levels of gamma interferon, interleukin-12
(IL-12) and tumor necrosis factor alpha. IL-10 was also decreased in
depleted animals. Additionally, splenic populations of CD4+
T cells, CD8+ T cells, and NK1.1+ cells were
decreased in depleted mice. Neutrophil-depleted mice exhibited lesions
of greater severity in tissues examined and a greater parasite burden
as determined by histopathology and reverse transcription-PCR. We
conclude that neutrophils are critical near the time of infection
because they influence the character of the immune response and control
tachyzoite replication.
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401. Phone: (607) 253-4022; Fax: (607)
253-3384. E-mail: eyd1{at}cornell.edu.
Infection and Immunity, August 2001, p. 4898-4905, Vol. 69, No. 8
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.8.4898-4905.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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