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Infection and Immunity, August 2001, p. 5157-5161, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.5157-5161.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Phagocytosis of Wild-Type Legionella pneumophila Occurs through a Wortmannin-Insensitive Pathway

Nadia Khelef,1,2,* Howard A. Shuman,3 and Frederick R. Maxfield1

Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 100211; Unité des Bordetella, Institut Pasteur, 75015 Paris, France2; and Department of Microbiology, Columbia University, New York, New York 100323

Received 27 December 2000/Returned for modification 7 February 2001/Accepted 8 May 2001

Wild-type Legionella pneumophila grows in human macrophages within a replicative phagosome, avoiding lysosomal fusion, while nonreplicative mutants are killed in lysosomes. Wortmannin, a phosphatidylinositol 3-kinase (PI3K) inhibitor, blocks phagocytosis of an avirulent mutant, but not of wild-type L. pneumophila, without affecting membrane ruffling and actin polymerization. These results show that wild-type and mutant Legionella strains use different entry pathways. They suggest that PI3Ks are involved in phagocytosis of an avirulent L. pneumophila mutant and regulate the ability of microorganisms to generate a replicative phagosome.

* Corresponding author. Present address: Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33.1.45.68.89.95. Fax: 33.1.45.68.87.06. E-mail: nkhelef{at}pasteur.fr.

Infection and Immunity, August 2001, p. 5157-5161, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.5157-5161.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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