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Infection and Immunity, August 2001, p. 5162-5165, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.5162-5165.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Localization of Surface Immunogenic Protein on Group B Streptococcus

Stéphane Rioux,1,* Denis Martin,1 Hans-Wolfgang Ackermann,2 Julie Dumont,1 Josée Hamel,1 and Bernard R. Brodeur1

Unité de Recherche en Vaccinologie, Centre Hospitalier Universitaire de Québec et Université Laval, Ste-Foy, Québec, Canada G1V 4G2,1 and Département de Biologie Médicale, Faculté de Médecine, Université Laval, Québec, Canada G1K 7P42

Received 26 January 2001/Returned for modification 6 March 2001/Accepted 1 May 2001

The localization and accessibility of the group B streptococcus (GBS) surface immunogenic protein (Sip) at the surface of intact GBS cells were studied by flow cytometric assay and immunogold electron microscopy. Antibodies present in pooled sera collected from mice after immunization with purified recombinant Sip efficiently recognized native Sip at the surfaces of the different GBS strains tested, which included representatives of all nine serotypes. Examination of GBS cells by immunogold electron microscopy revealed that the Sip-specific antibodies attached preferentially to polar sites and the septal region. This result confirmed that Sip is exposed at the intact-cell surface, but it also suggests that its distribution is restricted to certain regions of the cell.

* Corresponding author. Mailing address: Unité de Recherche en Vaccinologie, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Édifice T-367, 2705 Boul. Laurier, Ste-Foy, Québec, Canada G1V 4G2. Phone: (418) 656-4141, ext. 6259. Fax: (418) 654-2280. E-mail: Stephane.Rioux{at}crchul.ulaval.ca.

Infection and Immunity, August 2001, p. 5162-5165, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.5162-5165.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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