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Infection and Immunity, August 2001, p. 5198-5202, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.5198-5202.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification and Analysis of Staphylococcus aureus Components Expressed by a Model System of Growth in Serum

Michael D. Wiltshire and Simon J. Foster*

Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom

Received 29 January 2001/Returned for modification 16 March 2001/Accepted 25 April 2001

A model system mimicking Staphylococcus aureus bacteremia was developed by growth in serum under microaerobic conditions. Eight genes induced by growth in serum were identified, including an antimicrobial peptide biosynthesis locus, amino acid biosynthetic loci, and genes encoding putative surface proteins. Nine independent insertions were found in the major lysine biosynthesis operon, which encodes eight genes, is repressed by lysine in vitro, and is expressed in vivo.

* Corresponding author. Mailing address: Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom. Phone: 44 114 2224411. Phone: 44 114 2728697. E-mail: S.Foster{at}sheffield.ac.uk.

Infection and Immunity, August 2001, p. 5198-5202, Vol. 69, No. 8
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.8.5198-5202.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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