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Infection and Immunity, September 2001, p. 5230-5234, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5230-5234.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Predominance of Serotype-Specific Mucosal Antibody Response in Shigella flexneri-Infected Humans Living in an Area of Endemicity

Voahangy Rasolofo-Razanamparany,1 Anne-Marie Cassel-Beraud,2,dagger Jean Roux,1,2,Dagger Philippe J. Sansonetti,3 and Armelle Phalipon3,*

Unité d'Immunologie-Biologie Moléculaire1 and Unité de Recherches sur les Maladies Diarrhéiques,2 Institut Pasteur, BP1274 101 Antananarivo, Madagascar, and Unité de Pathogénie Microbienne Moléculaire, U389, Institut Pasteur, 75724 Paris Cedex 15, France3

Received 9 January 2001/Returned for modification 24 April 2001/Accepted 31 May 2001

The mucosal humoral immune response elicited following Shigella flexneri infection in patients living in Antananarivo districts (Madagascar Island) was evaluated by measuring the gut-derived, circulating immunoglobulin A (IgA) antibody-secreting cells (ASC) specific for the major bacterial antigen lipopolysaccharide (LPS). Fifty, 34, 11, and 5% of the S. flexneri-positive patients were infected with serotypes 2a, 1a, 4a, and 3a, respectively. The total number of IgA ASC in infected patients increased significantly, compared to the number in healthy controls, early after the onset of disease. The number of anti-homologous LPS IgA ASC varied among individuals and peaked between days 5 and 10 after the onset of the disease. In the S. flexneri 1a- and 2a-infected patients, the level of IgA ASC cross-reactivity to heterologous S. flexneri serotypes was weak. These data indicate that S. flexneri 2a and 1a are the predominant strains responsible for shigellosis in this area of endemicity and that the anti-LPS antibody response following natural infection is mainly directed against serotype-specific determinants.

* Corresponding author. Mailing address: Unité de Pathogénie Microbienne Moléculaire, U389, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex, France. Phone: 00 33 1 40 61 32 46. Fax: 00 33 1 45 68 89 53. E-mail: phalipon{at}pasteur.fr.

dagger Present address: Centre Muraz, BP 01 153 Bobodioulasso, Burkina Faso.

Dagger Present address: Institut Pasteur Paris, Délégation Générale du Réseau International des Instituts Pasteur et Instituts Associés, Paris, France.

Infection and Immunity, September 2001, p. 5230-5234, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5230-5234.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Phalipon, A., Costachel, C., Grandjean, C., Thuizat, A., Guerreiro, C., Tanguy, M., Nato, F., Vulliez-Le Normand, B., Belot, F., Wright, K., Marcel-Peyre, V., Sansonetti, P. J., Mulard, L. A. (2006). Characterization of Functional Oligosaccharide Mimics of the Shigella flexneri Serotype 2a O-Antigen: Implications for the Development of a Chemically Defined Glycoconjugate Vaccine. J. Immunol. 176: 1686-1694 [Abstract] [Full Text]  


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