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Infection and Immunity, September 2001, p. 5742-5751, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5742-5751.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

dksA Is Required for Intercellular Spread of Shigella flexneri via an RpoS-Independent Mechanism

Scott A. Mogull, Laura J. Runyen-Janecky, Mei Hong,dagger and Shelley M. Payne*

Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712-1095

Received 1 February 2001/Returned for modification 13 April 2001/Accepted 31 May 2001

Pathogenesis of Shigella flexneri is dependent on the ability of the bacterium to invade and spread within epithelial cells. In this study, we identified dksA as a gene necessary for intercellular spread in, but not invasion of, cultured cells. The S. flexneri dksA mutant exhibited sensitivity to acid and oxidative stress, in part due to an effect of DksA on production of RpoS. However, an S. flexneri rpoS mutant formed plaques on tissue culture monolayers, thus excluding DksA regulation of RpoS as the mechanism responsible for the inability of the dksA mutant to spread intercellularly. Intracellular analysis of the dksA mutant indicates that it survived and divided within the Henle cell cytoplasm, but the dksA mutant cells were elongated, and some exhibited filamentation in the intracellular environment. Some of the S. flexneri dksA mutant cells showed aberrant localization of virulence protein IcsA, which may inhibit spread between epithelial cells.

* Corresponding author. Mailing address: Section of Molecular Genetics and Microbiology, The University of Texas, Austin, TX 78712-1095. Phone: (512) 471-9258. Fax: (512) 471-7088. E-mail: payne{at}mail.utexas.edu.

dagger Present address: DoubleTwist Inc., Oakland, CA 94612.

Infection and Immunity, September 2001, p. 5742-5751, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5742-5751.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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