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Infection and Immunity, September 2001, p. 5940-5942, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5940-5942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cryptosporidium parvum Infection Requires Host Cell Actin Polymerization

David A. Elliott,1 Daniel J. Coleman,1 Michael A. Lane,1 Robin C. May,2 Laura M. Machesky,2 and Douglas P. Clark1,*

Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205,1 and School of Biosciences, Division of Molecular and Cell Biology, University of Birmingham, Birmingham, United Kingdom2

Received 16 February 2001/Returned for modification 4 April 2001/Accepted 28 May 2001

The intracellular protozoan parasite Cryptosporidium parvum accumulates host cell actin at the interface between the parasite and the host cell cytoplasm. Here we show that the actin polymerizing proteins Arp2/3, vasodilator-stimulated phosphoprotein (VASP), and neural Wiskott Aldrich syndrome protein (N-WASP) are present at this interface and that host cell actin polymerization is necessary for parasite infection.

* Corresponding author. Mailing address: The Johns Hopkins Hospital, 406 Pathology Building, 600 N. Wolfe St., Baltimore, MD 21287. Phone: (410) 955-1180. Fax: (410) 614-9556. E-mail: dclark{at}jhmi.edu.

Infection and Immunity, September 2001, p. 5940-5942, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5940-5942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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