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Infection and Immunity, September 2001, p. 5943-5948, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5943-5948.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation and Characterization of a Vibrio vulnificus Mutant Deficient in Both Extracellular Metalloprotease and Cytolysin

Jong-Jin Fan, Chung-Ping Shao, Ya-Chi Ho, Chun-Keung Yu, and Lien-I Hor*

Department of Microbiology and Immunology, College of Medicine, National Cheng-Kung University, Tainan 701, Taiwan, Republic of China

Received 5 March 2001/Returned for modification 10 May 2001/Accepted 19 June 2001

We isolated a Vibrio vulnificus mutant that was deficient in both metalloprotease and cytolysin by allelic exchange. The virulence of this mutant in mice and its cytotoxicity for HEp-2 cells were comparable to those of the wild-type strain, indicating that neither factor was essential for these properties. The cytolysin, but not the protease, seemed to be important for causing damage in the alimentary tract of the mice.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, College of Medicine, National Cheng-Kung University, Tainan 701, Taiwan, Republic of China. Phone: 886-6-2353535, ext. 5635. Fax: 886-6-2082705. E-mail: h061453{at}mail.ncku.edu.tw.

Infection and Immunity, September 2001, p. 5943-5948, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5943-5948.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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