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Infection and Immunity, September 2001, p. 5953-5957, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5953-5957.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of a Competitive Index Assay To Evaluate the Virulence of Listeria monocytogenes actA Mutants during Primary and Secondary Infection of Mice

Victoria Auerbuch,1 Laurel L. Lenz,1 and Daniel A. Portnoy1,2,*

Department of Molecular and Cell Biology1 and The School of Public Health,2 University of California, Berkeley, California 94720-3202

Received 17 January 2001/Returned for modification 26 March 2001/Accepted 5 June 2001

We developed a competitive index assay for murine listeriosis that tests the virulence of Listeria monocytogenes strains in different organs and at various times postinoculation. Studies presented here demonstrate the reproducibility of this assay during primary and secondary infection of inbred and outbred mice. We verified the validity of this assay by performing competitive index analysis of a well-characterized strain of L. monocytogenes lacking the actA gene. In addition, we found that while L. monocytogenes strains unable to recruit vasodilator-stimulated phosphoprotein (VASP) to their surface exhibit a 10-fold virulence attenuation in the livers of naive animals, they display a 50-fold survival defect in the liver during secondary listeriosis.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202. Phone: (510) 643-3925. Fax: (510) 643-6791. E-mail: portnoy{at}uclink4.berkeley.edu.

Infection and Immunity, September 2001, p. 5953-5957, Vol. 69, No. 9
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.9.5953-5957.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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