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Infect Immun. 1973 January; 7(1): 29-34
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Creatine Phosphokinase Activity in Rabbit Alveolar Macrophages

¹ Departments of Biochemistry and Medicine, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103

ABSTRACT

An enzyme is present in extracts of rabbit alveolar macrophage which can catalyze the reaction of creatine phosphate with adenosine diphosphate to form adenosine triphosphate and creatine. The enzyme is moderately activated by reduced glutathione, has a pH optimum between pH 6.5 and 7.0, and shows an absolute requirement for Mg²⁺. The K_m for creatine phosphate is approximately 3.6 mM while the K_m for adenosine diphosphate is about 1.1 mM. The enzyme may play a role in the energy balance of the cell by creating a reserve of energy in the form of creatine phosphate.