This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Blachman, U. Articles by Pickett, M. J. Search for Related Content PubMed PubMed Citation Articles by Blachman, U. Articles by Pickett, M. J.

 Previous Article  |  Next Article 

Infect Immun. 1973 January; 7(1): 53-61
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Radiobacteriolysis: a New Technique Using Chromium-51 for Assaying Anti- Vibrio cholerae Antibodies

Departments of Bacteriology and Biology, University of California, Los Angeles, California 90024

ABSTRACT

A new method for detecting and quantitating antibodies against Vibrio cholerae is described. The reaction involves the release of radiochromium from prelabeled vibrios in the presence of specific antibody and complement. The entire assay can be completed within 5 hr. The method is highly reproducible, immunologically specific, temperature- and complement-dependent, and significantly more sensitive than other methods currently used for titration of anti-Vibrio cholerae antibodies. The technique is also potentially applicable to titration of antibodies against other gram-negative bacteria.