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Infect Immun. 1973 March; 7(3): 315-321
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of the ß Antigen of Neisseria gonorrhoeae

¹ Department of Medicine, State University of New York at Buffalo, Buffalo, New York 14215

ABSTRACT

The ß antigen of Neisseria gonorrhoeae has been isolated from the alkaline-extracted gonococcal endotoxin by ion exchange, molecular sieve, and powder block electrophoretic chromatography. Hemagglutination inhibition studies indicate that the preparation is essentially free of common enterobacterial and gonococcal antigen. Analysis of the isolated antigen by immunodiffusion and acrylamide gel electrophoresis reveal only one detectable component. Chemical studies indicate that the antigen is an acidic glycoprotein composed primarily of four major amino acids: alanine, glutamic acid, glycine, and proline. The antigen has an s_0,w of 8.55, and spectral analysis in the ultraviolet and visible range reveals a single absorption peak at 217 nm.