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Infect Immun. 1973 March; 7(3): 356-360
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms

¹ Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195

ABSTRACT

Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.

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