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Infect Immun. 1973 March; 7(3): 398-402
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Host Cell Range and Growth Characteristics of Bovine Parvoviruses ¹

^a Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80521

ABSTRACT

In assessing the host cell range of bovine parvoviruses, these viruses were found to replicate optimally in actively dividing bovine fetal lung and spleen cells. Other primary bovine fetal cells supported growth to a lesser extent, but bovine line cells and line cells of other animal species tested did not. Minimal infectivity remained after passage of bovine parvovirus in cells from chicken embryos and guinea pig fetuses. During bovine parvovirus replication in bovine fetal lung and spleen cells, production kinetics of infectious virus and hemagglutinins were determined. An eclipse period of 16 h occurred, and viral release from cells was not detected until 30 h after inoculation of bovine fetal lung cells and 36 h after inoculation of bovine fetal spleen cells. Cell-associated virus titers were always higher than extracellular virus titers. Hemagglutinins were detected in parallel to infectious virus.

² Present address: Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Va. 24061.

¹ This publication is scientific paper no. 1799 of the Colorado Agricultural Experiment Station.