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Infect Immun. 1973 March; 7(3): 487-492
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Immunosorbent for the Isolation of Specific Antibodies Against Mannan: Localization of Antigens in Yeast Cell Walls

¹ Research and Development Department, Nestlé Products Technical Assistance Ltd., 1800 Vevey, Switzerland

ABSTRACT

A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.

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