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Infection and Immunity, January 2002, p. 134-139, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.134-139.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Long-Range Mapping of the Streptococcus agalactiae Phylogenetic Lineage Restriction Digest Pattern Type III-3 Reveals Clustering of Virulence Genes

John F. Bohnsack,1* April A. Whiting,1 Russell D. Bradford,1 Brenna K. Van Frank,1 Shinji Takahashi,2 and Elisabeth E. Adderson3

Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City, Utah 84132,1 Division of Microbiology, Joshi-Eiyoh University, Chiyoda, Sakado, Saitama 350-0288, Japan,2 Department of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, Tennessee 381053

Received 10 July 2001/ Returned for modification 10 September 2001/ Accepted 18 October 2001

Human isolates of serotype III Streptococcus agalactiae (group B streptococcus [GBS]) can be divided into three separate phylogenetic lineages based on analysis of the restriction digest patterns (RDPs) of chromosomal DNA. Nine DNA sequences that are present in all isolates of the RDP III-3 phylogenetic lineage, but not in the other lineages, were identified by genomic subtractive hybridization. A complete physical map of a III-3 chromosome was constructed. Six of the nine III-3-specific sequences mapped to a 340-kb Sse8387I fragment which contains or is located close to known GBS virulence genes. One of the III-3-specific probes, AW-10, encodes part of GBSi1, a group II intron that is inserted at two sites within the GBS genome. The second chromosomal site for GBSi1 was isolated, sequenced, and mapped to a location near the locus responsible for hemolysin production. These findings suggest that the genetic variation that distinguishes the RDP type III-3 strains from other serotype III strains occurs largely within localized areas of the genome containing known or putative virulence genes.

* Corresponding author. Mailing address: Department of Pediatrics, University of Utah Health Sciences Center, 50 North Medical Dr., Salt Lake City, UT 84132. Phone: (801) 581-5319. Fax: (801) 585-9314. E-mail: john.bohnsack{at}hsc.utah.edu.

Editor: V. J. DiRita

Infection and Immunity, January 2002, p. 134-139, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.134-139.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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