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Infection and Immunity, January 2002, p. 199-203, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.199-203.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of the Murine T-Lymphocyte Response to Salmonella enterica Serovar Typhimurium Infection

Department of Immunology, Max-Planck-Institute for Infection Biology, 10117 Berlin, Germany

Received 22 March 2001/ Returned for modification 19 June 2001/ Accepted 16 October 2001

Infection of mice with Salmonella enterica serotype Typhimurium induces a strong Th1 cell response that is central for the control of infection. We infected mice of a resistant background with a virulent strain of S. enterica serovar Typhimurium and analyzed the kinetics and magnitude of the T-cell response. After infection, the majority of CD4⁺ and CD8⁺ splenocytes acquired an activated phenotype, as indicated by expression levels of CD44 and CD62L. In addition, after 3 to 4 weeks of infection, more than 20% of the CD4⁺ and more than 30% of the CD8⁺ T cells produced gamma interferon (IFN-) in response to short-term polyclonal stimulation. In contrast, we detected only a moderate (two- to threefold) expansion of both T-cell populations, and BrdU incorporation revealed that there was either no or only a limited increase in the in vivo proliferation of CD4⁺ and CD8⁺ T cells, respectively. Our results indicate that although an unexpectedly large population of both CD4⁺ and CD8⁺ T cells is activated and acquires the potential to secrete IFN-, this activation is not paralleled by substantial expansion of these T-cell populations.

Editor: A. D. O’Brien

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