This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, X.
Right arrow Articles by Mobley, H. L. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, X.
Right arrow Articles by Mobley, H. L. T.

 Previous Article  |  Next Article 

Infection and Immunity, January 2002, p. 389-394, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.389-394.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Visualization of Proteus mirabilis within the Matrix of Urease-Induced Bladder Stones during Experimental Urinary Tract Infection

Xin Li,1 Hui Zhao,1,{dagger} C. Virginia Lockatell,2 Cinthia B. Drachenberg,3 David E. Johnson,2,4 and Harry L. T. Mobley1*

Department of Microbiology and Immunology,1 Division of Infectious Diseases, Department of Medicine,2 Department of Pathology, University of Maryland School of Medicine,3 Veterans Affairs Medical Center, Baltimore, Maryland 212014

Received 2 July 2001/ Returned for modification 4 September 2001/ Accepted 4 October 2001

The virulence of a urease-negative mutant of uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using a CBA mouse model of catheterized urinary tract infection. Overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type P. mirabilis. At a high inoculum, the urease-negative mutant successfully colonized bladders of catheterized mice but did not cause urolithiasis and was still severely attenuated in its ability to ascend to kidneys. Using confocal laser scanning microscopy and scanning electron microscopy, we demonstrated the presence of P. mirabilis within the urease-induced stone matrix. Alizarin red S staining was used to detect calcium-containing deposits in bladder and kidney tissues of P. mirabilis-infected mice.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Maryland School of Medicine, 655 W. Baltimore Street, Room 13-009, Baltimore, MD 21201. Phone: (410) 706-0466. Fax: (410) 706-6751. E-mail: hmobley{at}umaryland.edu.

Editor: D. L. Burns

{dagger} Present address: Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305.

Infection and Immunity, January 2002, p. 389-394, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.389-394.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nielubowicz, G. R., Smith, S. N., Mobley, H. L. T. (2008). Outer Membrane Antigens of the Uropathogen Proteus mirabilis Recognized by the Humoral Response during Experimental Murine Urinary Tract Infection. Infect. Immun. 76: 4222-4231 [Abstract] [Full Text]  
  • Pearson, M. M., Sebaihia, M., Churcher, C., Quail, M. A., Seshasayee, A. S., Luscombe, N. M., Abdellah, Z., Arrosmith, C., Atkin, B., Chillingworth, T., Hauser, H., Jagels, K., Moule, S., Mungall, K., Norbertczak, H., Rabbinowitsch, E., Walker, D., Whithead, S., Thomson, N. R., Rather, P. N., Parkhill, J., Mobley, H. L. T. (2008). Complete Genome Sequence of Uropathogenic Proteus mirabilis, a Master of both Adherence and Motility. J. Bacteriol. 190: 4027-4037 [Abstract] [Full Text]  
  • Jacobsen, S. M., Stickler, D. J., Mobley, H. L. T., Shirtliff, M. E. (2008). Complicated Catheter-Associated Urinary Tract Infections Due to Escherichia coli and Proteus mirabilis. Clin. Microbiol. Rev. 21: 26-59 [Abstract] [Full Text]  
  • Belzer, C, Kusters, J G, Kuipers, E J, van Vliet, A H M (2006). Urease induced calcium precipitation by Helicobacter species may initiate gallstone formation.. Gut 55: 1678-1679 [Full Text]  
  • Burall, L. S., Harro, J. M., Li, X., Lockatell, C.V., Himpsl, S. D., Hebel, J. R., Johnson, D. E., Mobley, H. L. T. (2004). Proteus mirabilis Genes That Contribute to Pathogenesis of Urinary Tract Infection: Identification of 25 Signature-Tagged Mutants Attenuated at Least 100-Fold. Infect. Immun. 72: 2922-2938 [Abstract] [Full Text]  
  • Li, X., Lockatell, C. V., Johnson, D. E., Lane, M. C., Warren, J. W., Mobley, H. L. T. (2004). Development of an Intranasal Vaccine To Prevent Urinary Tract Infection by Proteus mirabilis. Infect. Immun. 72: 66-75 [Abstract] [Full Text]  
  • Poore, C. A., Mobley, H. L. T. (2003). Differential regulation of the Proteus mirabilis urease gene cluster by UreR and H-NS. Microbiology 149: 3383-3394 [Abstract] [Full Text]  
  • Cieslikowski, T., Gradecka, D., Mielczarek, M., Kaca, W. (2003). Tandem Tetramer-Based Microsatellite Fingerprinting for Typing of Proteus mirabilis Strains. J. Clin. Microbiol. 41: 1673-1680 [Abstract] [Full Text]  
  • Dattelbaum, J. D., Lockatell, C. V., Johnson, D. E., Mobley, H. L. T. (2003). UreR, the Transcriptional Activator of the Proteus mirabilis Urease Gene Cluster, Is Required for Urease Activity and Virulence in Experimental Urinary Tract Infections. Infect. Immun. 71: 1026-1030 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»