This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Uhlich, G. A.
Right arrow Articles by Elder, R. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uhlich, G. A.
Right arrow Articles by Elder, R. O.

 Previous Article  |  Next Article 

Infection and Immunity, January 2002, p. 395-399, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.395-399.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Variations in the csgD Promoter of Escherichia coli O157:H7 Associated with Increased Virulence in Mice and Increased Invasion of HEp-2 Cells

Gaylen A. Uhlich,* James E. Keen, and Robert O. Elder,{dagger}

Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933

Received 26 April 2001/ Returned for modification 24 July 2001/ Accepted 9 October 2001

Promoter alterations in the csgD gene of Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 are associated with variations in curli expression and the ability to bind Congo red dye. Red variants of each strain were more invasive for cultured HEp-2 cells than were white variants. An ATCC 43895 red variant was more virulent than a white variant in a mouse model. However, there were no differences in Shiga toxin production between red and white variants.

* Corresponding author. Present address: USDA, ARS, Eastern Regional Research Center, 600 East Mermaid Ln., Wyndmoor, PA 19038. Phone: (215) 233-6740. Fax: (215) 233-6581. E-mail: guhlich{at}arserrc.gov.

Editor: B. B. Finlay

{dagger} Present address: USDA, ARS, Southern Plains Agricultural Research Center, College Station, TX 77845.

Infection and Immunity, January 2002, p. 395-399, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.395-399.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Uhlich, G. A., Gunther, N. W. IV, Bayles, D. O., Mosier, D. A. (2009). The CsgA and Lpp Proteins of an Escherichia coli O157:H7 Strain Affect HEp-2 Cell Invasion, Motility, and Biofilm Formation. Infect. Immun. 77: 1543-1552 [Abstract] [Full Text]  
  • Xicohtencatl-Cortes, J., Monteiro-Neto, V., Saldana, Z., Ledesma, M. A., Puente, J. L., Giron, J. A. (2009). The Type 4 Pili of Enterohemorrhagic Escherichia coli O157:H7 Are Multipurpose Structures with Pathogenic Attributes. J. Bacteriol. 191: 411-421 [Abstract] [Full Text]  
  • Dong, T., Coombes, B. K., Schellhorn, H. E. (2009). Role of RpoS in the Virulence of Citrobacter rodentium. Infect. Immun. 77: 501-507 [Abstract] [Full Text]  
  • Rosser, T., Dransfield, T., Allison, L., Hanson, M., Holden, N., Evans, J., Naylor, S., La Ragione, R., Low, J. C., Gally, D. L. (2008). Pathogenic Potential of Emergent Sorbitol-Fermenting Escherichia coli O157:NM. Infect. Immun. 76: 5598-5607 [Abstract] [Full Text]  
  • Torres, A. G., Slater, T. M., Patel, S. D., Popov, V. L., Arenas-Hernandez, M. M. P. (2008). Contribution of the Ler- and H-NS-Regulated Long Polar Fimbriae of Escherichia coli O157:H7 during Binding to Tissue-Cultured Cells. Infect. Immun. 76: 5062-5071 [Abstract] [Full Text]  
  • Dyer, J. G., Sriranganathan, N., Nickerson, S. C., Elvinger, F. (2007). Curli Production and Genetic Relationships Among Escherichia coli from Cases of Bovine Mastitis. J DAIRY SCI 90: 193-201 [Abstract] [Full Text]  
  • Uhlich, G. A., Cooke, P. H., Solomon, E. B. (2006). Analyses of the Red-Dry-Rough Phenotype of an Escherichia coli O157:H7 Strain and Its Role in Biofilm Formation and Resistance to Antibacterial Agents. Appl. Environ. Microbiol. 72: 2564-2572 [Abstract] [Full Text]  
  • Torres, A. G., Jeter, C., Langley, W., Matthysse, A. G. (2005). Differential Binding of Escherichia coli O157:H7 to Alfalfa, Human Epithelial Cells, and Plastic Is Mediated by a Variety of Surface Structures. Appl. Environ. Microbiol. 71: 8008-8015 [Abstract] [Full Text]  
  • Bokranz, W., Wang, X., Tschape, H., Romling, U. (2005). Expression of cellulose and curli fimbriae by Escherichia coli isolated from the gastrointestinal tract. J Med Microbiol 54: 1171-1182 [Abstract] [Full Text]  
  • Dziva, F., van Diemen, P. M., Stevens, M. P., Smith, A. J., Wallis, T. S. (2004). Identification of Escherichia coli O157 : H7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis. Microbiology 150: 3631-3645 [Abstract] [Full Text]  
  • Torres, A. G., Kaper, J. B. (2003). Multiple Elements Controlling Adherence of Enterohemorrhagic Escherichia coli O157:H7 to HeLa Cells. Infect. Immun. 71: 4985-4995 [Abstract] [Full Text]  
  • Persson, K., Russell, W., Morgelin, M., Herwald, H. (2003). The Conversion of Fibrinogen to Fibrin at the Surface of Curliated Escherichia coli Bacteria Leads to the Generation of Proinflammatory Fibrinopeptides. J. Biol. Chem. 278: 31884-31890 [Abstract] [Full Text]  
  • Shaikh, N., Tarr, P. I. (2003). Escherichia coli O157:H7 Shiga Toxin-Encoding Bacteriophages: Integrations, Excisions, Truncations, and Evolutionary Implications. J. Bacteriol. 185: 3596-3605 [Abstract] [Full Text]  
  • Chirwa, N. T., Herrington, M. B. (2003). CsgD, a regulator of curli and cellulose synthesis, also regulates serine hydroxymethyltransferase synthesis in Escherichia coli K-12. Microbiology 149: 525-535 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»