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Infection and Immunity, January 2002, p. 79-85, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.79-85.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of a Promiscuous T-Cell Epitope in Mycobacterium tuberculosis Mce Proteins

Maddalena Panigada,1 Tiziana Sturniolo,2 Giorgio Besozzi,3 Maria Giovanna Boccieri,4 Francesco Sinigaglia,2 Giuliana Gialdroni Grassi,5 and Fabio Grassi1*

Dipartimento di Biologia e Genetica per le Scienze Mediche, Università di Milano, 20133 Milan,1 Roche Milano Ricerche, 20132 Milan,2 Consorzio Antitubercolare "Istituto Villa Marelli," 20100 Milan,3 Azienda Ospedaliera "S. Maria degli Angeli," 33170 Pordenone,4 Cattedra di Chemioterapia, Università di Pavia, 27100 Pavia, Italy5

Received 16 July 2001/ Returned for modification 27 August 2001/ Accepted 16 October 2001

The characterization of Mycobacterium tuberculosis antigens inducing CD4+ T-cell responses could critically contribute to the development of subunit vaccines for M. tuberculosis. Here we performed computational analysis by using T-cell epitope prediction software (known as TEPITOPE) to predict promiscuous HLA-DR ligands in the products of the mce genes of M. tuberculosis. The analysis of the proliferative responses of CD4+ T cells from patients with pulmonary tuberculosis to selected peptides displaying promiscuous binding to HLA-DR in vitro led us to the identification of a peptide that induced proliferation of CD4+ cells from 50% of the tested subjects. This study demonstrates that a systematic computational approach can be used to identify T-cell epitopes in proteins expressed by an intracellular pathogen.

* Corresponding author. Present address: Harvard Medical School, Dana Farber Cancer Institute, Smith Building, Room 736, 1 Jimmy Fund Way, Boston MA 02115. Phone: (617) 632-6885. Fax: (617) 632-6881. E-mail: fabio_grassi{at}dfci.harvard.edu.

Editor: W. A. Petri, Jr.

Infection and Immunity, January 2002, p. 79-85, Vol. 70, No. 1
0019-9567/01/$04.00+0     DOI: 10.1128/IAI.70.1.79-85.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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