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Infection and Immunity, October 2002, p. 5363-5369, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5363-5369.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Immune Responses to Novel Pneumococcal Proteins Pneumolysin, PspA, PsaA, and CbpA in Adenoidal B Cells from Children

Qibo Zhang,1* Sharon Choo,2 and Adam Finn1

Institute of Child Health, University of Bristol, UBHT Education Centre, Bristol BS2 8AE, United Kingdom,1 Department of Immunology, Princess Margaret Hospital for Children, Subiaco, Western Australia 6008, Australia2

Received 21 March 2002/ Returned for modification 22 May 2002/ Accepted 10 June 2002

Studies of mice suggest that pneumococcal proteins, including PspA, pneumolysin, PsaA, and CbpA, are promising vaccine candidates. To determine whether these proteins are good mucosal immunogens in humans, adenoidal lymphocytes from 20 children who had adenoidectomies were isolated and tested by ELISpot for antigen-specific antibody-secreting cells (ASCs). Cells were also cultured for 7 days in the presence of a concentrated culture supernatant (CCS) from a type 14 strain of pneumococcus which contained secreted pneumococcal proteins, including PspA, pneumolysin, PsaA, and CbpA, and then tested by ELISpot. ELISpot assays done on freshly isolated cells detected ASCs to all four antigens in most children studied. However, there were differences both between antigens and between isotypes. The densities of immunoglobulin G (IgG) ASCs against both PsaA and CbpA were significantly higher than those of ASCs for PspA and PdB (pneumolysin toxoid B) (P < 0.001). For all antigens, the numbers of IgA ASCs tended to be lower than those of both IgG and IgM ASCs. The numbers of anti-CbpA and -PsaA IgA ASCs were higher than those of anti-PdB IgA ASCs (P < 0.01). Concentrations of IgA antibodies to PspA and PsaA in saliva correlated with the numbers of IgA ASCs to PspA and PsaA in freshly isolated adenoidal cells, but no such correlation was found between salivary IgG antibody concentrations and IgG ASCs to the four antigens in adenoidal cells. In cultured cells, anti-PspA, -PsaA, and -CbpA IgG ASCs proliferated significantly, but only two of eight samples showed >2-fold increases in anti-CbpA and -PspA IgA ASCs after CCS stimulation. The results suggest that CbpA, PsaA, and PspA may be good upper respiratory mucosal antigens in children. Adenoids may be important inductive sites for memory IgG responses and important sources of salivary IgA. Some protein antigens may also prime for mucosal IgA memory. These data support the effort to explore mucosal immunization against pneumococcal infection.

* Corresponding author. Mailing address: Institute of Child Health, University of Bristol, UBHT Education Centre, Upper Maudlin St., Bristol BS2 8AE, United Kingdom. Phone: (0044) (0) 117 3420199. Fax: (0044) (0) 117 3420178. E-mail: q.zhang{at}bristol.ac.uk.

Editor: E. I. Tuomanen

Infection and Immunity, October 2002, p. 5363-5369, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5363-5369.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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