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Infection and Immunity, October 2002, p. 5503-5511, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5503-5511.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Representational Difference Analysis between Afa/Dr Diffusely Adhering Escherichia coli and Nonpathogenic E. coli K-12

Anne-Beatrice Blanc-Potard,1 Colin Tinsley,2 Isabel Scaletsky,3 Chantal Le Bouguenec,4 Julie Guignot,1 Alain L. Servin,1* Xavier Nassif,2 and Marie-Francoise Bernet-Camard1

Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 510, Faculté de Pharmacie Paris XI, 92296 ChÂtenay-Malabry,1 INSERM U411, Faculté de Médecine Necker-Enfants Malades, 75730 Paris,2 Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 75724 Paris, France,4 Department of Microbiology, Immunology, and Parasitology, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo, Brazil3

Received 24 April 2002/ Accepted 25 June 2002

Diffusely adhering Escherichia coli strains harboring Afa/Dr adhesins (Afa/Dr DAEC) have been associated with diarrhea and urinary tract infections (UTIs). The present work is the first extensive molecular study of a Afa/Dr DAEC strain using the representational difference analysis technique. We have searched for DNA sequences present in strain C1845, recovered from a diarrheagenic child, but absent from a nonpathogenic K-12 strain. Strain C1845 harbors part of a pathogenicity island (PAICFT073) and several iron transport systems found in other E. coli pathovars. We did not find genes encoding factors known to subvert host cell proteins, such as type III secretion system or effector proteins. Several C1845-specific sequences are homologous to putative virulence genes or show no homology with known sequences, and we have analyzed their distribution among Afa/Dr and non-Afa/Dr clinical isolates and among strains from the E. coli Reference Collection. Three C1845-specific sequences (MO30, S109, and S111) have a high prevalence (77 to 80%) among Afa/Dr strains and a low prevalence (12 to 23%) among non-Afa/Dr strains. In addition, our results indicate that strain IH11128, an Afa/Dr DAEC strain recovered from a patient with a UTI, is genetically closely related to strain C1845.

* Corresponding author. Mailing address: INSERM Unité 510, UFR de Pharmacie Paris XI, F-92296 ChÂtenay-Malabry, France. Phone: 33 1 46 83 56 61. Fax: 33 1 46 83 58 44. E-mail: alain.servin{at}cep.u-psud.fr.

Editor: V. J. DiRita

Infection and Immunity, October 2002, p. 5503-5511, Vol. 70, No. 10
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.10.5503-5511.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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